Mouse HtrA serine peptidase 1 ELISA Kit | HTRA1 elisa kit

Mouse Serine protease HTRA1, HTRA1 ELISA Kit

Cat. Num. AAA906562

• Gene Names: Htra1; L56; HTRA; Prss11; RSPP11; AI429470
• Reactivity: Mouse
• Synonyms: HtrA serine peptidase 1; Mouse Serine protease HTRA1; HTRA1 ELISA Kit; Mouse Serine protease HTRA1 (HTRA1) ELISA kit; ARMD7; HtrA; L56; ORF480; PRSS11; IGFBP5-protease; protease; serine; 11 (IGF binding) ; HTRA1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of rat HTR1A. No significant cross-reactivity or interference between rat HTR1A and analogues was observed.
• Sequence Length: 480

• Samples: Serum, Plasma, Tissue Homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 23.5 pg/ml-1500 pg/ml
• Sensitivity: 5.8 pg/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HTRA1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HTR1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HTR1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HTR1A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HTR1A bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 229093139
• NCBI GeneID: 56213
• NCBI Accession #: NP_062510.2
• NCBI GenBank Nucleotide #: NM_019564.3
• UniProt Accession #: Q9R118; Q8BN04; Q8BN05; Q91WS3; Q9QZK6
• Molecular Weight: 51,214 Da
• NCBI Official Full Name: serine protease HTRA1
• NCBI Official Synonym Full Names: HtrA serine peptidase 1
• NCBI Official Symbol: Htra1
• NCBI Official Synonym Symbols: L56; HTRA; Prss11; RSPP11; AI429470
• NCBI Protein Information: serine protease HTRA1; serine protease 11; protease, serine, 11 (Igf binding); high-temperature requirement A serine peptidase 1; insulin-like growth factor binding protein 5 protease
• UniProt Protein Name: Serine protease HTRA1
• Protein Family: Serine protease
• UniProt Gene Name: Htra1
• UniProt Synonym Gene Names: Htra; Prss11
• UniProt Entry Name: HTRA1_MOUSE

Uniprot Description

HTRA1: Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, although it is unclear whether TGF-beta proteins are themselves degraded. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets. Variations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7). ARMD is the leading cause of vision loss and blindness among older individuals in the developed word. It is classified as either dry (nonneovascular) or wet (neovascular). ARMD7 is a wet form, in which new blood vessels form and break beneath the retina. This leakage causes permanent damage to surrounding retinal tissue, distorting and destroying central vision. Wet ARMD is more prevalent among Asians than Caucasians. Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL). CARASIL is characterized by nonhypertensive cerebral small-vessel arteriopathy with subcortical infarcts, alopecia, and spondylosis, with an onset in early adulthood. On neuropathological examination, atherosclerosis associated with intimal thickening and dense collagen fibers, loss of vascular smooth-muscle cells, and hyaline degeneration of the tunica media has been observed in cerebral small arteries. Belongs to the peptidase S1B family.

Protein type: Secreted, signal peptide; EC 3.4.21.-; Protease; Secreted

Cellular Component: extracellular matrix; cytoplasm; extracellular region

Molecular Function: peptidase activity; insulin-like growth factor binding; protein binding; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; growth factor binding; catalytic activity

Biological Process: regulation of cell growth; negative regulation of defense response to virus; negative regulation of transforming growth factor beta receptor signaling pathway; proteolysis; positive regulation of epithelial cell proliferation; negative regulation of BMP signaling pathway