Human Serine protease HTRA1 ELISA Kit | HTRA1 elisa kit

Human Serine protease HTRA1 ELISA Kit

Cat. Num. AAA765316

• Gene Names: HTRA1; L56; HtrA; ARMD7; ORF480; PRSS11; CARASIL; CADASIL2
• Reactivity: Human
• Synonyms: Serine protease HTRA1; Human Serine protease HTRA1 ELISA Kit; HTRA1 (Serine protease HTRA1)/IGFBP5-protease/PRSS11/ARMD7/High-temperature requirement A serine peptidase 1/HtrA serine peptidase 1/L56/ORF480/Serine protease 11/serine; 11 (IGF binding)/CARASIL/HtrA; HTRA1 elisa kit

• Reactivity: Human
• Sequence Length: 480

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for HTRA1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human HTRA1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human HTRA1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human HTRA1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human HTRA1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4506141
• NCBI GeneID: 5654
• NCBI Accession #: NP_002766.1
• NCBI GenBank Nucleotide #: NM_002775.4
• Molecular Weight: 51,287 Da
• NCBI Official Full Name: serine protease HTRA1
• NCBI Official Synonym Full Names: HtrA serine peptidase 1
• NCBI Official Symbol: HTRA1
• NCBI Official Synonym Symbols: L56; HtrA; ARMD7; ORF480; PRSS11; CARASIL; CADASIL2
• NCBI Protein Information: serine protease HTRA1
• UniProt Protein Name: Serine protease HTRA1
• Protein Family: Serine protease
• UniProt Gene Name: HTRA1
• UniProt Synonym Gene Names: HTRA; PRSS11
• UniProt Entry Name: HTRA1_HUMAN

NCBI Description

This gene encodes a member of the trypsin family of serine proteases. This protein is a secreted enzyme that is proposed to regulate the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. It has also been suggested to be a regulator of cell growth. Variations in the promoter region of this gene are the cause of susceptibility to age-related macular degeneration type 7. [provided by RefSeq, Jul 2008]

Uniprot Description

HTRA1: Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, although it is unclear whether TGF-beta proteins are themselves degraded. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets. Variations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7). ARMD is the leading cause of vision loss and blindness among older individuals in the developed word. It is classified as either dry (nonneovascular) or wet (neovascular). ARMD7 is a wet form, in which new blood vessels form and break beneath the retina. This leakage causes permanent damage to surrounding retinal tissue, distorting and destroying central vision. Wet ARMD is more prevalent among Asians than Caucasians. Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL). CARASIL is characterized by nonhypertensive cerebral small-vessel arteriopathy with subcortical infarcts, alopecia, and spondylosis, with an onset in early adulthood. On neuropathological examination, atherosclerosis associated with intimal thickening and dense collagen fibers, loss of vascular smooth-muscle cells, and hyaline degeneration of the tunica media has been observed in cerebral small arteries. Belongs to the peptidase S1B family.

Protein type: Secreted; Protease; Secreted, signal peptide; EC 3.4.21.-

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: cytosol; extracellular matrix; extracellular space; plasma membrane

Molecular Function: insulin-like growth factor binding; serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: negative regulation of BMP signaling pathway; negative regulation of defense response to virus; negative regulation of transforming growth factor beta receptor signaling pathway; placenta development; positive regulation of epithelial cell proliferation; proteolysis; regulation of cell growth

Disease: Cerebral Autosomal Recessive Arteriopathy With Subcortical Infarcts And Leukoencephalopathy; Macular Degeneration, Age-related, 7