Human HtrA Serine Peptidase 1 (HTRA1) RTU ELISA Kit | HTRA1 rtu elisa kit

Human HtrA Serine Peptidase 1 (HTRA1) ELISA Kit

Cat. Num. AAA459997

• Gene Names: HTRA1; L56; HtrA; ARMD7; ORF480; PRSS11; CARASIL; CADASIL2
• Reactivity: Human
• Synonyms: HtrA Serine Peptidase 1 (HTRA1); Human HtrA Serine Peptidase 1 (HTRA1) ELISA Kit; HtrA; L56; ORF480; PRSS11; HtrA Serine Peptidase 1; Protease; Serine; 11; IGFBP5-Protease; High-temperature requirement A serine peptidase 1; HTRA1 rtu elisa kit

• Reactivity: Human
• Sequence Length: 480

• Assay Type: Sandwich
• Samples: Serum, Plasma or Other Biological Fluids.
• Detection Range: 31.2-2000pg/mL
• Sensitivity: 13.2pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HTRA1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HTRA1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to HTRA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HTRA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HTRA1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4506141
• NCBI GeneID: 5654
• NCBI Accession #: NP_002766.1
• NCBI GenBank Nucleotide #: NM_002775.4
• UniProt Accession #: Q92743; Q9UNS5; D3DRE4
• Molecular Weight: 51,287 Da
• NCBI Official Full Name: serine protease HTRA1
• NCBI Official Synonym Full Names: HtrA serine peptidase 1
• NCBI Official Symbol: HTRA1
• NCBI Official Synonym Symbols: L56; HtrA; ARMD7; ORF480; PRSS11; CARASIL; CADASIL2
• NCBI Protein Information: serine protease HTRA1
• UniProt Protein Name: Serine protease HTRA1
• Protein Family: Serine protease
• UniProt Gene Name: HTRA1
• UniProt Synonym Gene Names: HTRA; PRSS11

NCBI Description

This gene encodes a member of the trypsin family of serine proteases. This protein is a secreted enzyme that is proposed to regulate the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. It has also been suggested to be a regulator of cell growth. Variations in the promoter region of this gene are the cause of susceptibility to age-related macular degeneration type 7. [provided by RefSeq, Jul 2008]

Uniprot Description

Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, although it is unclear whether TGF-beta proteins are themselves degraded. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets.