Western Blot (WB)
(Western blot analysis of PPIC Antibody (Center) in Hela cell line lysates (35ug/lane). PPIC (arrow) was detected using the purified Pab.)
Rabbit anti-Human PPIC Polyclonal Antibody | anti-PPIC antibody
PPIC, ID (PPIC, CYPC, Peptidyl-prolyl cis-trans isomerase C, Cyclophilin C, Rotamase C) (APC)
Gene Names
PPIC; CYPC
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
PPIC; Polyclonal Antibody; ID (PPIC; CYPC; Peptidyl-prolyl cis-trans isomerase C; Cyclophilin C; Rotamase C) (APC); Rotamase C; anti-PPIC antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-PPIC antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
PPIC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68~97 amino acids from the Center region of human PPIC.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western blot analysis of PPIC Antibody (Center) in Hela cell line lysates (35ug/lane). PPIC (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of PPIC Antibody (Center) in Hela cell line lysates (35ug/lane). PPIC (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with PPIC Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with PPIC Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(PPIC Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(PPIC Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-PPIC antibody
PPIC is a member of the peptidyl-prolyl cis-trans isomerase (PPIase)) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. Similar to other PPIases, this protein can bind immunosuppressant cyclosporin A.
Product Categories/Family for anti-PPIC antibody
References
Vasan, R.S. JAMA 302 (2), 168-178 (2009). Lim, J. Cell 125 (4), 801-814 (2006). Trahey, M. Proc. Natl. Acad. Sci. U.S.A. 96 (6), 3006-3011 (1999).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,763 Da
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase C
NCBI Official Synonym Full Names
peptidylprolyl isomerase C
NCBI Official Symbol
PPIC
NCBI Official Synonym Symbols
CYPC
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase C
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase C
Protein Family
UniProt Gene Name
PPIC
UniProt Synonym Gene Names
CYPC; PPIase C
UniProt Entry Name
PPIC_HUMAN
Uniprot Description
PPIC: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Belongs to the cyclophilin-type PPIase family.
Protein type: Cyclophilin; EC 5.2.1.8; Isomerase
Chromosomal Location of Human Ortholog: 5q23.2
Molecular Function: cyclosporin A binding; peptidyl-prolyl cis-trans isomerase activity; protein binding
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Product Notes
The PPIC ppic (Catalog #AAA6336259) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PPIC, ID (PPIC, CYPC, Peptidyl-prolyl cis-trans isomerase C, Cyclophilin C, Rotamase C) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PPIC can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IHC: 1:50-100 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PPIC ppic for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPIC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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