Typical Testing Data/Standard Curve (for reference only)
Rat Heat shock 70 kDa protein 1A/1B ELISA Kit | Hspa1a elisa kit
Rat Heat shock 70 kDa protein 1A/1B ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat shock 70 kDa protein 1A/1B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forHeat shock 70 kDa protein 1A/1B and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Heat shock 70 kDa protein 1A/1B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Heat shock 70 kDa protein 1A/1B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
heat inducible gene; induced by global ischemia and kainic acid-induced seizures [RGD, Feb 2006]
Uniprot Description
HSP70: a critical chaperone protein that has a high affinity for unfolded polypeptide chains. It binds extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In cooperation with other chaperones, hsp70 stabilizes preexistent proteins against aggregation and mediates the folding of newly translated polypeptides in the cytosol as well as within organelles. Mitochondrial HSP70 is crucial to the import process: mutant forms of HSP70 fail to import precursor proteins. Is anti-apoptotic in sympathetic neurones and mediates this effect primarily by suppressing c-Jun transcriptional signalling. Interacts with tau protein and mediates proper folding of tau. Can promote the degradation of tau protein. Triptolide, a potential therapeutic agent for progression/metastasis of pancreatic cancer, causes pancreatic cancer cell death by induction of apoptosis, an effect mediated by the inhibition of HSP70.
Protein type: Heat shock protein; Motility/polarity/chemotaxis; Chaperone
Cellular Component: apical plasma membrane; basolateral plasma membrane; centriole; cytoplasm; cytosol; focal adhesion; inclusion body; intracellular; mitochondrion; nucleus; perinuclear region of cytoplasm; protein complex; ubiquitin ligase complex
Molecular Function: ATP binding; ATPase activity; ATPase activity, coupled; enzyme binding; G-protein-coupled receptor binding; heat shock protein binding; histone deacetylase binding; NF-kappaB binding; protease binding; protein binding; receptor binding; ubiquitin protein ligase binding; unfolded protein binding
Biological Process: activation of NF-kappaB transcription factor; ATP metabolic process; binding of sperm to zona pellucida; defense response; negative regulation of apoptosis; negative regulation of caspase activity; negative regulation of myeloid cell apoptosis; negative regulation of protein ubiquitination; negative regulation of vasoconstriction; positive regulation of interleukin-8 production; positive regulation of T cell mediated cytotoxicity; protein refolding; protein stabilization; regulation of protein ubiquitination; response to heat; response to unfolded protein
Research Articles on Hspa1a
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Product Notes
The Rat Hspa1a (Catalog #AAA2886253) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2886253 ELISA Kit recognizes Rat Hspa1a. It is sometimes possible for the material contained within the vial of "Heat shock 70 kDa protein 1A/1B, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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