Human Hemopexin ELISA Kit | HPX elisa kit

Human Hemopexin PicoKine ELISA Kit

Cat. Num. AAA178531

• Gene Names: HPX; HX
• Reactivity: Human
• Synonyms: Hemopexin; Human Hemopexin PicoKine ELISA Kit; Beta 1B glycoprotein; Beta-1B-glycoprotein; HEMO; Hpx; HX; P02790; HPX elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human Hemopexin
• Sequence Length: 462

• Samples: Cell culture supernates, serum and plasma(heparin, EDTA).
• Assay Type: Sandwich
• Detection Range: 3.12ng/ml-200ng/ml
• Sensitivity: <50pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).

Typical Testing Data/Standard Curve (for reference only)

(Human Hemopexin PicoKine ELISA Kit standard curve )

Related Product Information for HPX elisa kit

Principle of the Assay: Human Hemopexin ELISA Kit was based on standard sandwich enzyme-linked immunesorbent assay technology. A monoclonal antibody from mouse specific for Hemopexin has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: T24-H462) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Hemopexin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Hemopexin amount of sample captured in plate.

Background/Introduction: Hemopexin (or haemopexin; HPX), also known as beta-1B-glycoprotein, is a protein that in humans is encoded by the HPX gene and belongs to hemopexin family of proteins. Hemopexin binds heme with the highest affinity of any known protein. Its function is scavenging the heme released or lost by the turnover of heme proteins such as hemoglobin and thus protects the body from the oxidative damage that free heme can cause. In addition, hemopexin releases its bound ligand for internalisation upon interacting with a specific receptor situated on the surface of liver cells. This function of hemopexin is to preserve the body's iron.

NCBI and Uniprot Product Information

• NCBI GI #: 11321561
• NCBI GeneID: 3263
• NCBI Accession #: NP_000604.1
• NCBI GenBank Nucleotide #: NM_000613.2
• UniProt Accession #: P02790; B2R957
• Molecular Weight: 51,676 Da
• NCBI Official Full Name: hemopexin
• NCBI Official Synonym Full Names: hemopexin
• NCBI Official Symbol: HPX
• NCBI Official Synonym Symbols: HX
• NCBI Protein Information: hemopexin
• UniProt Protein Name: Hemopexin
• Protein Family: Hemopexin
• UniProt Gene Name: HPX
• UniProt Entry Name: HEMO_HUMAN

NCBI Description

This gene encodes a plasma glycoprotein that binds heme with high affinity. The encoded protein is an acute phase protein that transports heme from the plasma to the liver and may be involved in protecting cells from oxidative stress. [provided by RefSeq, Apr 2009]

Uniprot Description

HPX: Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation. Belongs to the hemopexin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p15.5-p15.4

Cellular Component: extracellular region; extracellular space

Molecular Function: protein binding

Biological Process: receptor-mediated endocytosis