Human Hemopexin ELISA Kit | HPX elisa kit

Human Hemopexin ELISA Kit

Cat. Num. AAA8248361

• Gene Names: HPX; HX
• Reactivity: Human
• Synonyms: Hemopexin; Human Hemopexin ELISA Kit; Beta-1B-glycoprotein; HPX elisa kit

• Reactivity: Human
• Specificity: The Human Hemopexin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Hemopexin proteins within the range of 3120 pg/ml - 200000 pg/ml.
• Sequence Length: 462

• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: <50 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HPX elisa kit

Principle of the Assay: The Human Hemopexin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Hemopexin in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Human Hemopexin coated on a 96-well plate. Standards and samples are pipetted into the wells and Hemopexin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Hemopexin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Hemopexin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Hemopexin (or haemopexin; HPX), also known as beta-1B-glycoprotein, is a protein that in humans is encoded by the HPX gene and belongs to hemopexin family of proteins. Hemopexin binds heme with the highest affinity of any known protein. Its function is scavenging the heme released or lost by the turnover of heme proteins such as hemoglobin and thus protects the body from the oxidative damage that free heme can cause. In addition, hemopexin releases its bound ligand for internalisation upon interacting with a specific receptor situated on the surface of liver cells. This function of hemopexin is to preserve the body's iron.

NCBI and Uniprot Product Information

• NCBI GI #: 11321561
• NCBI GeneID: 3263
• NCBI Accession #: NP_000604.1
• NCBI GenBank Nucleotide #: NM_000613.2
• UniProt Accession #: P02790; B2R957
• Molecular Weight: 51,676 Da
• NCBI Official Full Name: hemopexin
• NCBI Official Synonym Full Names: hemopexin
• NCBI Official Symbol: HPX
• NCBI Official Synonym Symbols: HX
• NCBI Protein Information: hemopexin
• UniProt Protein Name: Hemopexin
• Protein Family: Hemopexin
• UniProt Gene Name: HPX
• UniProt Entry Name: HEMO_HUMAN

NCBI Description

This gene encodes a plasma glycoprotein that binds heme with high affinity. The encoded protein is an acute phase protein that transports heme from the plasma to the liver and may be involved in protecting cells from oxidative stress. [provided by RefSeq, Apr 2009]

Uniprot Description

HPX: Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation. Belongs to the hemopexin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p15.5-p15.4

Cellular Component: extracellular region; extracellular space

Molecular Function: protein binding

Biological Process: receptor-mediated endocytosis