Glutathione Reductase Assay Kit | GSR assay kit

Glutathione Reductase Assay Kit

Cat. Num. AAA480419

• Gene Names: GSR; HEL-75
• Synonyms: Glutathione Reductase; Glutathione Reductase Assay Kit; Spectrophotometric Assay for Glutathione Reductase; GSR assay kit

• Sequence Length: 230

• Preparation and Storage: 4 degree C

Testing Data

Testing Data #2

Related Product Information for GSR assay kit

Principle of the Assay: This assay is based on the oxidation of NADPH to NADP+ catalyzed by a limiting concentration of glutathione reductase. One GR activity unit is defined as the amount of enzyme catalyzing the reduction of one micromole of GSSG per minute at pH 7.6 and 25 degree C. As shown in the above reaction, one molecule of NADPH is consumed for each molecule of GSSG reduced. Therefore, the reduction of GSSG is determined indirectly by the measurement of the consumption of NADPH, as demonstrated by a decrease in absorbance at 340 nm (A340) as a function of time.

References

1. Dolphin D, Poulson R, Avramovic O, Eds. (1989) Glutathione: Chemical, Biochemical and Metabolic Aspects, Vols. A and B, J. Wiley and Sons.
2. Bashir A, Perham RN, Scrutton NS, Berry A (1995) "Altering kinetic mechanism and enzyme stability by mutagenesis of the dimer interface of glutathione reductase" Biochem.J. 312, 527-533.
3. Massey V, Williams CH (1965) "On the mechanism of yeast glutathione reductase" J.Biol.Chem. 240 (11), 4470-4480.
4. Worthington DJ, Rosemeyer MA (1976) "Glutathione reductase from human erythrocytes. Catalytic properties and aggregation" Eur.J.Biochem. 67, 231-238.
5. García-Alfonso C, Martìnez-Galisteo, Llobell A, Bárcena JA, López-Barea J (1993) "Horse-liver glutathione reductase: purification and characterization" Int.J.Biochem. 25 (1), 61-68.
6. Carlberg I, Mannervik B (1985) "Glutathione reductase" Meth.Enzymol. 113, 485-490.
7. Peindo J, García-Alfonso C, Martínez-Galisteo E, Llobell A, López-Barea J (1991) "Metals are directly involved in the redox interconversion of Saccharomyces cervisiae glutathione reductase" Molec.Cell.Biol. 101, 175-187.
8. García-Alfonso C, Martínez-Galisteo E, Llobell A, Bácena J, López-Barea J (1993) "Horse-liver glutathione reductase: purification and characterization" Int.J.Biochem. 25 (4), 513-520.
9. Smith I, Vierheller T, Thorne C (1988) "Assay of glutathione reductase in crude tissue homogenates using 5,5'- dithiobis(2-nitrobenzoic acid)" Anal.Biochem. 75, 408-413.
10. Beutler, E (1969) "Effect of flavin compounds on the glutathione reductase activity: in vitro and in vivo studies" J.Clin.Invest. 48, 1957-1966.

NCBI and Uniprot Product Information

• NCBI GI #: 31829
• NCBI GeneID: 2936
• UniProt Accession #: Q03504; P00390
• Molecular Weight: 25,081 Da
• NCBI Official Full Name: glutathione reductase, partial
• NCBI Official Synonym Full Names: glutathione reductase
• NCBI Official Symbol: GSR
• NCBI Official Synonym Symbols: HEL-75
• NCBI Protein Information: glutathione reductase, mitochondrial; GR; GRase; epididymis luminal protein 75
• UniProt Protein Name: Glutathione reductase
• Protein Family: Glutathione reductase
• UniProt Entry Name: Q03504_HUMAN

NCBI Description

This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein. It is a central enzyme of cellular antioxidant defense, and reduces oxidized glutathione disulfide (GSSG) to the sulfhydryl form GSH, which is an important cellular antioxidant. Rare mutations in this gene result in hereditary glutathione reductase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2010]

Uniprot Description

GSR: a class-I pyridine nucleotide-disulfide oxidoreductase. Maintains high levels of reduced glutathione in the cytosol. Alternative initiation results in a mitochondrial and a cytoplasmic isoform.

Protein type: Oxidoreductase; EC 1.8.1.7; Other Amino Acids Metabolism - glutathione

Chromosomal Location of Human Ortholog: 8p21.1

Cellular Component: mitochondrial matrix; cytosol; external side of plasma membrane

Molecular Function: mercury (II) reductase activity; electron carrier activity; FAD binding; mercury ion binding; glutathione-disulfide reductase activity; NADP binding

Biological Process: response to reactive oxygen species; glutathione metabolic process; nucleobase, nucleoside and nucleotide metabolic process; cell redox homeostasis; nucleobase, nucleoside and nucleotide interconversion; detoxification of mercury ion