Rabbit AKAP8 Polyclonal Antibody | anti-AKAP8 antibody

AKAP8 Antibody

Cat. Num. AAA9504836

• Gene Names: AKAP8; AKAP-8; AKAP95; AKAP 95; AKAP-95
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Synonyms: AKAP8; Polyclonal Antibody; AKAP8 Antibody; A-kinase anchor protein 8; A-kinase anchor protein 95 kDa; AKA8; AKAP 95; AKAP95; anti-AKAP8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: AKAP8 Antibody detects endogenous levels of total AKAP8 protein.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 692

• Applicable Applications for anti-AKAP8 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:20000
• Target Modification: Unmodified/Total
• Immunogen: The antiserum was produced against synthesized peptide derived from human AKAP8.
• Immunogen Range: 331-380
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Immunofluorescence (IF)

(Immunofluorescence analysis of HUVEC cells, using AKAP8 Antibody. The picture on the right is blocked with the synthesized peptide.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human heart tissue, using AKAP8 Antibody. The picture on the right is blocked with the synthesized peptide.)

Western Blot (WB)

(Western blot analysis of lysates from mouse heart and Jurkat cells, using AKAP8 Antibody. The lane on the right is blocked with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 5031579
• NCBI GeneID: 10270
• NCBI Accession #: NP_005849.1
• NCBI GenBank Nucleotide #: NM_005858.3
• UniProt Accession #: O43823
• Molecular Weight: 76 kDa
• NCBI Official Full Name: A-kinase anchor protein 8
• NCBI Official Synonym Full Names: A-kinase anchoring protein 8
• NCBI Official Symbol: AKAP8
• NCBI Official Synonym Symbols: AKAP-8; AKAP95; AKAP 95; AKAP-95
• NCBI Protein Information: A-kinase anchor protein 8
• UniProt Protein Name: A-kinase anchor protein 8
• Protein Family: A-kinase anchor protein
• UniProt Gene Name: AKAP8
• UniProt Synonym Gene Names: AKAP95; AKAP-8; AKAP 95

NCBI Description

This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, May 2011]

Uniprot Description

Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II) (PubMed:9473338). Acts as an anchor for a PKA-signaling complex onto mitotic chromosomes, which is required for maintenance of chromosomes in a condensed form throughout mitosis. Recruits condensin complex subunit NCAPD2 to chromosomes required for chromatin condensation; the function appears to be independent from PKA-anchoring (PubMed:10601332, PubMed:10791967, PubMed:11964380). May help to deliver cyclin D/E to CDK4 to facilitate cell cycle progression (PubMed:14641107). Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3; in this function may act redundantly with AKAP8L (PubMed:16980585). Involved in nuclear retention of RPS6KA1 upon ERK activation thus inducing cell proliferation (PubMed:22130794). May be involved in regulation of DNA replication by acting as scaffold for MCM2 (PubMed:12740381). Enhances HMT activity of the KMT2 family MLL4/WBP7 complex and is involved in transcriptional regulation. In a teratocarcinoma cell line is involved in retinoic acid-mediated induction of developmental genes implicating H3 'Lys-4' methylation (PubMed:23995757). May be involved in recruitment of active CASP3 to the nucleus in apoptotic cells (PubMed:16227597). May act as a carrier protein of GJA1 for its transport to the nucleus (PubMed:26880274). Seems to involved in modulation of rDNA transcription. Preferentially binds GC-rich DNA in vitro and associates to GC-rich ribosomal RNA promoters (PubMed:26683827). Involved in modulation of Toll-like receptor signaling. Required for the cAMP-dependent suppression of TNF-alpha in early stages of LPS-induced macrophage activation; the function probably implicates targeting of PKA to NFKB1 ().