Chicken Hemopexin (HPX) ELISA Kit | HPX elisa kit

Gallus Hemopexin (HPX) ELISA Kit

Cat. Num. AAA2704011

• Gene Names: HPX; HX
• Reactivity: Chicken
• Synonyms: Hemopexin (HPX); Gallus Hemopexin (HPX) ELISA Kit; Haemopexin; Beta-1B-glycoprotein; HPX elisa kit

• Reactivity: Chicken
• Specificity: This assay has high sensitivity and excellent specificity for detection of Hemopexin (HPX).; No significant cross-reactivity or interference between Hemopexin (HPX) and analogues was observed.
• Sequence Length: 462

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 6.25-400ng/mL
• Sensitivity: < 2.69ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hemopexin (HPX) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hemopexin (HPX) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HPX elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hemopexin (HPX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hemopexin (HPX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hemopexin (HPX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hemopexin (HPX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 11321561
• NCBI GeneID: 3263
• NCBI Accession #: NP_000604.1
• NCBI GenBank Nucleotide #: NM_000613.2
• UniProt Accession #: P02790; B2R957
• Molecular Weight: 51,676 Da
• NCBI Official Full Name: hemopexin
• NCBI Official Synonym Full Names: hemopexin
• NCBI Official Symbol: HPX
• NCBI Official Synonym Symbols: HX
• NCBI Protein Information: hemopexin
• UniProt Protein Name: Hemopexin
• Protein Family: Hemopexin
• UniProt Gene Name: HPX

NCBI Description

This gene encodes a plasma glycoprotein that binds heme with high affinity. The encoded protein is an acute phase protein that transports heme from the plasma to the liver and may be involved in protecting cells from oxidative stress. [provided by RefSeq, Apr 2009]

Uniprot Description

HPX: Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation. Belongs to the hemopexin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: extracellular region; extracellular space

Molecular Function: heme transporter activity; metal ion binding; protein binding

Biological Process: cellular iron ion homeostasis; heme metabolic process; heme transport; hemoglobin metabolic process; positive regulation of humoral immune response mediated by circulating immunoglobulin; positive regulation of immunoglobulin production; positive regulation of tyrosine phosphorylation of STAT protein; receptor-mediated endocytosis; viral process