15-PGDH Recombinant Protein | HPGD recombinant protein

15-PGDH Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122802

• Gene Names: Hpgd; 15-PGDH; AV026552
• Purity: >90% as determined by SDS-PAGE
• Synonyms: 15-PGDH; 15-PGDH Protein; Mouse; Recombinant (His Tag); Mouse HPGD/15-PGDH Protein (His Tag); AV026552 Protein; hydroxyprostaglandin dehydrogenase 15-(NAD); HPGD recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH8.0, 20% glycerol. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser269

• Species: Mouse
• Predicted N Terminal: Met 1
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse HPGD (Q8VCC1)(Met 1-Ser 269) was expressed, with a polyhistide tag at the C-terusmin.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for HPGD recombinant protein

Background: 15-hydroxyprostaglandin dehydrogenase [NAD+], also known as Prostaglandin dehydrogenase 1, HPGD, and PGDH1, is a member of the short-chain dehydrogenases/reductases (SDR) family. Prostaglandins (PGs) play a key role in the onset of labor in many species and regulate uterine contractility and cervical dilatation. Therefore, the regulation of prostaglandin output by PG synthesizing and metabolizing enzymes in the human myometrium may determine uterine activity patterns in human labor both at preterm and at term. Prostaglandin dehydrogenase (PGDH) metabolizes prostaglandins (PGs) to render them inactive. HPGD is down-regulated by cortisol, dexamethasone, and betamethasone and down-regulated in colon cancer. It is up-regulated by TGFB1. HPGD contributes to the regulation of events that are under the control of prostaglandin levels. HPGD catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. and inhibits in vivo proliferation of colon cancer cells. Defects in HPGD are the cause of primary hypertrophic osteoarthropathy autosomal recessive (PHOAR), cranio-osteoarthropathy (COA), and isolated congenital nail clubbing.

References

Patel, FA. et al., 2003, J. Clin. Endocrinol. Metab. 88: 2922-33. McKeown KJ, et al.,2003, J. Clin. Endocrinol. Metab. 88 (4): 1737-41. Yan, M. et al., 2004, Proc. Natl. Acad. Sci. USA. 101: 17468-73. Tariq, M. et al., 2009, J Med Genet. 46 (1): 14-20.

NCBI and Uniprot Product Information

• NCBI GI #: 81901849
• NCBI GeneID: 15446
• UniProt Accession #: Q8VCC1; Q61106
• Molecular Weight: 29,181 Da
• NCBI Official Full Name: 15-hydroxyprostaglandin dehydrogenase
• NCBI Official Synonym Full Names: hydroxyprostaglandin dehydrogenase 15 (NAD)
• NCBI Official Symbol: Hpgd
• NCBI Official Synonym Symbols: 15-PGDH; AV026552
• NCBI Protein Information: 15-hydroxyprostaglandin dehydrogenase [NAD(+)]
• UniProt Protein Name: 15-hydroxyprostaglandin dehydrogenase [NAD(+)]
• Protein Family: 15-hydroxyprostaglandin dehydrogenase
• UniProt Gene Name: Hpgd
• UniProt Synonym Gene Names: Pgdh1; 15-PGDH

Uniprot Description

Prostaglandin inactivation. Contributes to the regulation of events that are under the control of prostaglandin levels. Catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4 ().