Mouse SRY Monoclonal Antibody | anti-SRY antibody

Anti-SRY Antibody

Cat. Num. AAA415144

• Applications: Western Blot, ELISA
• Synonyms: SRY; Monoclonal Antibody; Anti-SRY Antibody; anti-SRY antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: SRY.G12
• Specificity: Recognize mouse SRY protein, others not tested.
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 204

• Applicable Applications for anti-SRY antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: Western blot (1:1000) and ELISA (1:2000~5000). The Sex-Determining Region (SRY) is a gene that encodes a transcription factor that is a member of the high mobility group HMG-box family of DNA binding proteins.The gene is located on the short arm of the Y chromosome and has a gene map locus - Yp11.3.Common synonyms of the gene name are Testis Determining Factor (TDF) and Testis Determining Factor on Y (TDY). The main function of the SRY protein is to initiate male sex determination as a transcriptional activator, which regulates a genetic switch in male development. The SRY HMG box recognizes DNA by partial intercalation in the minor groove. The SRY protein then binds to the DNA and distorts its shape (SRY protein in action). In doing so, it regulates genes that control the development of the testes.
• Storage Buffer: PBS, pH 7.4 with 0.05% sodium azide.
• Immunogen: SRY-GST fusion protein.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot (WB)

Related Product Information for anti-SRY antibody

This intronless gene encodes a transcription factor that is a member of the high mobility group (HMG)-box family of DNA-binding proteins. This protein is the testis-determining factor (TDF), which initiates male sex determination. Mutations in this gene give rise to XY females with gonadal dysgenesis (Swyer syndrome); translocation of part of the Y chromosome containing this gene to the X chromosome causes XX male syndrome. [provided by RefSeq, Jul 2008]

NCBI and Uniprot Product Information

• NCBI GI #: 383087991
• Molecular Weight: 23,884 Da
• NCBI Official Full Name: SRY
• UniProt Protein Name: Sex-determining region Y protein
• Protein Family: SRY-related protein
• UniProt Gene Name: SRY
• UniProt Entry Name: A7WPU8_HUMAN

NCBI Description

This intronless gene encodes a transcription factor that is a member of the high mobility group (HMG)-box family of DNA-binding proteins. This protein is the testis-determining factor (TDF), which initiates male sex determination. Mutations in this gene give rise to XY females with gonadal dysgenesis (Swyer syndrome); translocation of part of the Y chromosome containing this gene to the X chromosome causes XX male syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

SRY: Transcriptional regulator that controls a genetic switch in male development. It is necessary and sufficient for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells. In male adult brain involved in the maintenance of motor functions of dopaminergic neurons. Involved in different aspects of gene regulation including promoter activation or repression. Promotes DNA bending. SRY HMG box recognizes DNA by partial intercalation in the minor groove. Also involved in pre-mRNA splicing. Binds to the DNA consensus sequence 5'-[AT]AACAA[AT]-3'. Defects in SRY are the cause of 46,XY sex reversal type 1 (SRXY1). A condition characterized by male-to-female sex reversal in the presence of a normal 46,XY karyotype. Patients manifest rapid and early degeneration of their gonads, which are present in the adult as 'streak gonads', consisting mainly of fibrous tissue and variable amounts of ovarian stroma. As a result these patients do not develop secondary sexual characteristics at puberty. The external genitalia in these subjects are completely female, and Muellerian structures are normal. A 45,X chromosomal aberration involving SRY is found in Turner syndrome, a disease characterized by gonadal dysgenesis with short stature, streak gonads, variable abnormalities such as webbing of the neck, cubitus valgus, cardiac defects, low posterior hair line. The phenotype is female. Defects in SRY are the cause of 46,XX sex reversal type 1 (SRXX1). A condition in which male gonads develop in a genetic female (female to male sex reversal). Belongs to the SRY family.

Protein type: Nuclear receptor co-regulator; DNA-binding

Chromosomal Location of Human Ortholog: Yp11.3

Cellular Component: cytoplasm; nuclear speck; nucleus

Molecular Function: calmodulin binding; RNA polymerase II transcription factor activity, enhancer binding; DNA binding; transcription factor binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; male sex determination; positive regulation of transcription, DNA-dependent; cell differentiation; sex differentiation

Disease: 46,xx Sex Reversal 1; 46,xy Sex Reversal 1