Rabbit anti-Mouse 15-PGDH Monoclonal Antibody | anti-HPGD antibody

Recombinant Anti-15-PGDH Antibody, Rabbit Monoclonal

Cat. Num. AAA8111704

• Gene Names: Hpgd; 15-PGDH; AV026552
• Reactivity: Mouse
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A
• Synonyms: 15-PGDH; Monoclonal Antibody; Recombinant Anti-15-PGDH Antibody; Rabbit Monoclonal; HPGD/15-PGDH Antibody; Rabbit MAb; hydroxyprostaglandin dehydrogenase 15-(NAD); Anti-15-PGDH Antibody; Anti-AV026552 Antibody; anti-HPGD antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 101
• Specificity: Mouse HPGD/15-PGDH
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-HPGD antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IP: 1-4uL/mg of lysate
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Mouse HPGD/15-PGDH protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse HPGD/15-PGDH (rM HPGD/15-PGDH).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-HPGD rabbit monoclonal antibody at 1:500 dilutionLane A: LOVO Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:29 kDaObserved band size:27 kDa)

Immunoprecipitation (IP)

(Mouse HPGD was immunoprecipitated using:Lane A:0.5 mg Caco-2 Whole Cell Lysate2 uL anti-Mouse HPGD rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse HPGD rabbit monoclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 29 kDaObserved band size: 29 kDa)

Related Product Information for anti-HPGD antibody

15-hydroxyprostaglandin dehydrogenase [NAD+], also known as Prostaglandin dehydrogenase 1, HPGD, and PGDH1, is a member of the short-chain dehydrogenases/reductases (SDR) family. Prostaglandins (PGs) play a key role in the onset of labor in many species and regulate uterine contractility and cervical dilatation. Therefore, the regulation of prostaglandin output by PG synthesizing and metabolizing enzymes in the human myometrium may determine uterine activity patterns in human labor both at preterm and at term. Prostaglandin dehydrogenase (PGDH) metabolizes prostaglandins (PGs) to render them inactive. HPGD is down-regulated by cortisol, dexamethasone, and betamethasone and down-regulated in colon cancer. It is up-regulated by TGFB1. HPGD contributes to the regulation of events that are under the control of prostaglandin levels. HPGD catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. and inhibits in vivo proliferation of colon cancer cells. Defects in HPGD are the cause of primary hypertrophic osteoarthropathy autosomal recessive (PHOAR), cranio-osteoarthropathy (COA), and isolated congenital nail clubbing.

References

Patel, FA. et al., 2003, J. Clin. Endocrinol. Metab. 88: 2922-33. McKeown KJ, et al., 2003, J. Clin. Endocrinol. Metab. 88 (4): 1737-41. Yan, M. et al., 2004, Proc. Natl. Acad. Sci. USA. 101: 17468-73. Tariq, M. et al., 2009, J Med Genet. 46 (1): 14-20.

NCBI and Uniprot Product Information

• NCBI GI #: 81901849
• NCBI GeneID: 15446
• UniProt Accession #: Q8VCC1; Q61106
• Molecular Weight: 29,181 Da
• NCBI Official Full Name: 15-hydroxyprostaglandin dehydrogenase
• NCBI Official Synonym Full Names: hydroxyprostaglandin dehydrogenase 15 (NAD)
• NCBI Official Symbol: Hpgd
• NCBI Official Synonym Symbols: 15-PGDH; AV026552
• NCBI Protein Information: 15-hydroxyprostaglandin dehydrogenase [NAD(+)]
• UniProt Protein Name: 15-hydroxyprostaglandin dehydrogenase [NAD(+)]
• Protein Family: 15-hydroxyprostaglandin dehydrogenase
• UniProt Gene Name: Hpgd
• UniProt Synonym Gene Names: Pgdh1; 15-PGDH

Uniprot Description

Prostaglandin inactivation. Contributes to the regulation of events that are under the control of prostaglandin levels. Catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4 ().