HMGB1/HMG1 Recombinant Protein | Hmgb1 recombinant protein

Human CellExp HMGB1/HMG1, Mouse Recombinant

Cat. Num. AAA8400088

• Gene Names: Hmgb1; p30; Hmg1; HMG-1; SBP-1
• Purity: >90% by SDS-PAGE
• Synonyms: HMGB1/HMG1; Human CellExp HMGB1/HMG1; Mouse Recombinant; HMGB1; HMG1; HMG3; SBP-1; Hmgb1 recombinant protein

• Host: HEK293 cells
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Lyophilized powder. Lyophilized from 0.22um filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Sequence: Met1-Glu215

• Gene Source: Mouse
• Endotoxin: Less than 1.0 EU per ug by the LAL method.
• Reconstitution Instruction: Reconstitute in sterile deionized water to the desired protein concentration.
• Tag Note: This protein carries a polyhistidine tag at the C-terminus.
• Preparation and Storage: Store at -20 degree C; Centrifuge the vial prior to opening.; Shelf life: ~12 months

SDS-Page

Related Product Information for Hmgb1 recombinant protein

High-mobility group protein B1 (HMGB1) is also known as high-mobility group protein 1 (HMG-1) and amphoterin, is a member of the HMGB family consisting of three members, HMGB1, HMGB2 and HMGB3. HMGB1 is a non-histone architectural chromosomal protein ubiquitously present in all vertebrate nuclei and binds double-stranded DNA without sequence specificity. The mechanism of inflammation and damage is binding to TLR4, which mediates HMGB1-dependent activation of macrophage cytokine release. This positions HMGB1 at the intersection of sterile and infectious inflammatory responses. HMGB1 has been studied as a DNA vaccine adjuvant and a target for cancer therapy.

Product Categories/Family for Hmgb1 recombinant protein

Proteins; Enzymes; Proteins & Enzymes (A-Z); Immunology; Inflammation Proteins; Human cell expressed recombinant proteins

NCBI and Uniprot Product Information

• NCBI GI #: 926657659
• NCBI GeneID: 15289
• NCBI Accession #: NP_001300823.1
• NCBI GenBank Nucleotide #: NM_001313894.1
• UniProt Accession #: P63158; P07155; P27109; P27428
• Molecular Weight: 24,894 Da
• NCBI Official Full Name: high mobility group protein B1
• NCBI Official Synonym Full Names: high mobility group box 1
• NCBI Official Symbol: Hmgb1
• NCBI Official Synonym Symbols: p30; Hmg1; HMG-1; SBP-1
• NCBI Protein Information: high mobility group protein B1
• UniProt Protein Name: High mobility group protein B1
• Protein Family: High mobility group protein
• UniProt Gene Name: Hmgb1
• UniProt Synonym Gene Names: Hmg-1; Hmg1; HMG-1

NCBI Description

This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]

Uniprot Description

Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy. Acts as danger associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury. Released to the extracellular environment can bind DNA, nucleosomes, IL-1 beta, CXCL12, AGER isoform 2/sRAGE, lipopolysaccharide (LPS) and lipoteichoic acid (LTA), and activates cells through engagement of multiple surface receptors. In the extracellular compartment fully reduced HMGB1 (released by necrosis) acts as a chemokine, disulfide HMGB1 (actively secreted) as a cytokine, and sulfonyl HMGB1 (released from apoptotic cells) promotes immunological tolerance (PubMed:23519706, PubMed:23446148, PubMed:23994764, PubMed:25048472). Has proangiogenic activity (PubMed:16365390). May be involved in platelet activation. Binds to phosphatidylserine and phosphatidylethanolamide. Bound to RAGE mediates signaling for neuronal outgrowth. May play a role in accumulation of expanded polyglutamine (polyQ) proteins ().