HMGB1/HMG1 Active Protein | HMGB1 active protein

Human CellExp HMGB1 / HMG1, human recombinant

Cat. Num. AAA844990

• Gene Names: HMGB1; HMG1; HMG3; SBP-1
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: HMGB1/HMG1; Human CellExp HMGB1 / HMG1; human recombinant; HMGB1; HMG1; HMG3; SBP-1; HMGB1 active protein

• Host: HEK 293 cells (Met1-Glu 215)
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Lyophilized from 0.22 um filtered solution in PBS. Trehalose (5%) was added as a protectant before lyophilization.
• Sequence Length: 215

• Applicable Applications for HMGB1 active protein: SDS-PAGE; HPLC
• Endotoxin Level: <1 EU/ug by LAL method
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4. Do not vortex. Store the solution in aliquots at -20°C for long term storage.
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized human AGER-Fc tag protein (100 ul/well) can bind to HMGB1 in a linear range of 0.31-2.5 ug/ml.
• Preparation and Storage: Store at -20°C. After reconstitution, aliquot and store at -20°C and use within 3 months. Avoid repeated freezing and thawing.

SDS-PAGE

(SDS-PAGE (4-20%) of Recombinant HMGB1: Recombinant protein loaded under reducing conditions and stained with Coomassie Blue. The protein shows a predicted MW of 32 kDa)

ELISA

(Biological activity: Measured by its binding ability in a functional ELISA HMGB1 is binding to immobilized human AGER-Fc tag protein (100 ug/well) with a linear range of 0.31-2.5 ug/ml.)

Related Product Information for HMGB1 active protein

High-mobility group protein B1 (HMGB1), also known as high-mobility group protein 1 (HMG-1) and amphoterin, is a member of the HMGB family consisting of three members, HMGB1, HMGB2 and HMGB3. HMGB1 is a non-histone architectural chromosomal protein ubiquitously present in all vertebrate nuclei and binds double-stranded DNA without sequence specificity. The mechanism of inflammation and damage is binding to TLR4, which mediates HMGB1-dependent activation of macrophage cytokine release. This positions HMGB1 at the intersection of sterile and infectious inflammatory responses. HMGB1 has been studied as a DNA vaccine adjuvant and a target for cancer therapy.

Product Categories/Family for HMGB1 active protein

Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteins Proteins and Enzymes; Human cell expressed recombinant proteins Proteins and Enzymes; Proteins and Enzymes (A-Z) Cytokines; Growth Factors & Hormones; Cytokines & Growth Facto

NCBI and Uniprot Product Information

• NCBI GI #: 4504425
• NCBI GeneID: 3146
• NCBI Accession #: NP_002119.1
• NCBI GenBank Nucleotide #: NM_002128.4
• UniProt Accession #: P09429; Q14321; Q5T7C3; Q6IBE1; A5D8W9
• Molecular Weight: The protein is fused with 6×His tag at the C-terminus, has a calculated MW of 25 kDa. The predicted N-terminus is Met1. DTT-reduced Protein migrates as 32 kDa and 35 kDa due to glycosylation.
• NCBI Official Full Name: high mobility group protein B1
• NCBI Official Synonym Full Names: high mobility group box 1
• NCBI Official Symbol: HMGB1
• NCBI Official Synonym Symbols: HMG1; HMG3; SBP-1
• NCBI Protein Information: high mobility group protein B1
• UniProt Protein Name: High mobility group protein B1
• Protein Family: High mobility group protein
• UniProt Gene Name: HMGB1
• UniProt Synonym Gene Names: HMG1; HMG-1
• UniProt Entry Name: HMGB1_HUMAN

Uniprot Description

HMGB1: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Belongs to the HMGB family.

Protein type: Nuclear receptor co-regulator; DNA repair, damage

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: nucleoplasm; extracellular space; cell surface; transcriptional repressor complex; extracellular region; condensed chromosome; nucleus

Molecular Function: protein binding; RAGE receptor binding; double-stranded DNA binding; damaged DNA binding; cytokine activity; chromatin binding; transcription factor activity; transcription factor binding; DNA bending activity; single-stranded DNA binding; chemoattractant activity

Biological Process: negative regulation of transcriptional preinitiation complex assembly; DNA topological change; V(D)J recombination; positive regulation of apoptosis; apoptosis; positive regulation of caspase activity; base-excision repair, DNA ligation; negative regulation of transcription from RNA polymerase II promoter; DNA recombination; regulation of transcription from RNA polymerase II promoter; chromatin remodeling; myeloid dendritic cell activation; inflammatory response to antigenic stimulus; positive chemotaxis; dendritic cell chemotaxis; innate immune response; DNA ligation during DNA repair; positive regulation of transcription from RNA polymerase II promoter; DNA fragmentation during apoptosis; neurite development; cell structure disassembly during apoptosis; positive regulation of DNA binding