High Mobility Group Protein 1 (HMGB1) Protein | HMGB1 protein

Eukaryotic High Mobility Group Protein 1 (HMGB1)

Cat. Num. AAA2124280

• Gene Names: Hmgb1; p30; Hmg1; HMG-1; SBP-1
• Applications: SDS-Page, Western Blot
• Purity: >90%
• Synonyms: High Mobility Group Protein 1 (HMGB1); Eukaryotic High Mobility Group Protein 1 (HMGB1); High Mobility Group Protein 1; HMG1; HMG3; SBP1; Sulfoglucuronyl Carbohydrate Binding Protein; Amphoterin; High Mobility Group Box 1 Protein; HMGB1 protein

• Host: 293F cells
• Purity/Purification: >90%
• Form/Format: Freeze-dried powder; 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Sequence: Met1~Glu215

• Applicable Applications for HMGB1 protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Source: Eukaryotic expression
• Species: Mouse
• Tag: N-terminal His Tag
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Isoelectric Point: 5.4
• Subcellular Location: Secreted
• Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

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SDS-Page

(Figure. SDS-PAGE)

Product Categories/Family for HMGB1 protein

Infection immunity; Eukaryotic proteins/EP

NCBI and Uniprot Product Information

• NCBI GI #: 926657659
• NCBI GeneID: 15289
• NCBI Accession #: NP_001300823.1
• NCBI GenBank Nucleotide #: NM_001313894.1
• UniProt Accession #: P63158; P07155; P27109; P27428
• Molecular Weight: 24,894 Da
• NCBI Official Full Name: high mobility group protein B1
• NCBI Official Synonym Full Names: high mobility group box 1
• NCBI Official Symbol: Hmgb1
• NCBI Official Synonym Symbols: p30; Hmg1; HMG-1; SBP-1
• NCBI Protein Information: high mobility group protein B1
• UniProt Protein Name: High mobility group protein B1
• Protein Family: High mobility group protein
• UniProt Gene Name: Hmgb1
• UniProt Synonym Gene Names: Hmg-1; Hmg1; HMG-1

NCBI Description

This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]

Uniprot Description

Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy. Acts as danger associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury. Released to the extracellular environment can bind DNA, nucleosomes, IL-1 beta, CXCL12, AGER isoform 2/sRAGE, lipopolysaccharide (LPS) and lipoteichoic acid (LTA), and activates cells through engagement of multiple surface receptors. In the extracellular compartment fully reduced HMGB1 (released by necrosis) acts as a chemokine, disulfide HMGB1 (actively secreted) as a cytokine, and sulfonyl HMGB1 (released from apoptotic cells) promotes immunological tolerance (PubMed:23519706, PubMed:23446148, PubMed:23994764, PubMed:25048472). Has proangiogenic activity (PubMed:16365390). May be involved in platelet activation. Binds to phosphatidylserine and phosphatidylethanolamide. Bound to RAGE mediates signaling for neuronal outgrowth. May play a role in accumulation of expanded polyglutamine (polyQ) proteins ().