SDS-Page
(Figure 1. Human CD47, mFc-His Tag on SDS-PAGE under reducing condition.)
CD47 protein
Human CD47 Protein, mFc-His Tag
Purity
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Synonyms
CD47; Human CD47 Protein; mFc-His Tag; MER6; IAP; OA3; CD47 protein
Host
HEK293 cells
Purity/Purification
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Form/Format
Lyophilized from sterile PBS, pH 7.4. Normally 5 %-15 % trehalose is added as protectants before lyophilization.
Sequence Positions
CD47 (Gln19-Gln141)+CD47 (Val198-Ala207)+CD47 (Ser257-Gly268)+mFc (Pro99-Lys330)
Species
Human
Vector and Tag
C-Mouse Fc and 6xHis tag
Preparation and Storage
Store at -80 degree C for 12 months (Avoid repeated freezing and thawing)
SDS-Page
(Figure 1. Human CD47, mFc-His Tag on SDS-PAGE under reducing condition.)
SDS-Page
(Figure 1. Human CD47, mFc-His Tag on SDS-PAGE under reducing condition.)
ELISA (EIA)
(Figure 2. ELISA plate pre-coated by 2ug/ml (100ul/well) Human CD47, mFc-His tagged protein can bind its native ligand Human SIRPalpha, hFc-His tagged protein (PME100009) in a linear range of 3.3-26.37ng/ml.)
ELISA (EIA)
(Figure 2. ELISA plate pre-coated by 2ug/ml (100ul/well) Human CD47, mFc-His tagged protein can bind its native ligand Human SIRPalpha, hFc-His tagged protein (PME100009) in a linear range of 3.3-26.37ng/ml.)
ELISA (EIA)
(Figure 3. ELISA plate pre-coated by 2ug/ml (100ul/well) Human CD47, mFc-His tagged protein can bind Anti-CD47 (Neutralizing antibody clone Hu5F9-G4) in a linear range of 0.061-1.606ng/ml.)
ELISA (EIA)
(Figure 3. ELISA plate pre-coated by 2ug/ml (100ul/well) Human CD47, mFc-His tagged protein can bind Anti-CD47 (Neutralizing antibody clone Hu5F9-G4) in a linear range of 0.061-1.606ng/ml.)
Related Product Information for CD47 protein
Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.
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Product Notes
The CD47 (Catalog #AAA8575066) is a Protein produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is CD47 (Gln19-Gln141)+CD47 (Val198-Ala207)+CD47 (Ser257-Gly268)+mFc (Pro99-Lys330). It is sometimes possible for the material contained within the vial of "CD47, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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