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Cd47 active protein

Human CellExp CD47, Fc Tag, Mouse Recombinant

Gene Names
Cd47; IAP; Itgp; AA407862; AI848868; AW108519; 9130415E20Rik; B430305P08Rik
Purity
>95% as determined by SDS-PAGE
Synonyms
CD47; Human CellExp CD47; Fc Tag; Mouse Recombinant; MER6; IAP; OA3; Cd47 active protein
Ordering
For Research Use Only!
Host
HEK293 cells
Purity/Purification
>95% as determined by SDS-PAGE
Form/Format
Lyophilized powder. Lyophilized from 0.22um filtered solution in 50mM Tris, 100mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization
Sequence
aaGln19-Lys140
Gene Source
Murine
Endotoxin
Less than 0.1 EU per ug by the LAL method.
Biological Activity
Immobilized Mouse CD47, Mouse IgG2a Fc Tag at 10 ug/mL (100 uL/well) can bind Biotinylated Human SIRP alpha, Fc Tag with a linear range of 0.16-5 ug/mL
Reconstitution Instruction
Reconstitute in sterile deionized water to a concentration of 100 ug/ml. Do not vortex. It is recommended to store at store at -20 degree C.
Tag Note
This protein carries a mouse IgG2a Fc tag at the C-terminus.
Preparation and Storage
Store at -20 degree C
Centrifuge the vial prior to opening.
Shelf life: ~12 months

SDS-Page

SDS-Page

Testing Data

Testing Data
Related Product Information for Cd47 active protein
Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. Plays a role in cell adhesion modulation of integrins

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33,098 Da
NCBI Official Full Name
leukocyte surface antigen CD47
NCBI Official Synonym Full Names
CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
NCBI Official Symbol
Cd47
NCBI Official Synonym Symbols
IAP; Itgp; AA407862; AI848868; AW108519; 9130415E20Rik; B430305P08Rik
NCBI Protein Information
leukocyte surface antigen CD47; Rh-related antigen; integrin-associated protein
UniProt Protein Name
Leukocyte surface antigen CD47
Protein Family
UniProt Gene Name
Cd47
UniProt Synonym Gene Names
IAP
UniProt Entry Name
CD47_MOUSE

Uniprot Description

CD47: Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: membrane; integral to plasma membrane; plasma membrane; integral to membrane

Molecular Function: protein binding

Biological Process: cell migration; response to bacterium; positive regulation of phagocytosis; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; opsonization; positive regulation of T cell activation; signal transduction; cell adhesion; positive regulation of inflammatory response

Research Articles on Cd47

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Product Notes

The Cd47 cd47 (Catalog #AAA8400014) is an Active Protein produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: aaGln19-Ly s140. It is sometimes possible for the material contained within the vial of "CD47, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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