Transcription Initiation Factor IIB/TFIIB/GTF2B Recombinant Protein | TFIIB/GTF2B recombinant protein

Recombinant Human TFIIB/GTF2B Protein (GST Tag)

Cat. Num. AAA2553305

• Gene Names: GTF2B; TF2B; TFIIB
• Purity: > 85 % as determined by reducing SDS-PAGE.
• Synonyms: Transcription Initiation Factor IIB/TFIIB/GTF2B; Recombinant Human TFIIB/GTF2B Protein (GST Tag); Transcription Initiation Factor IIB; General Transcription Factor TFIIB; S300-II; GTF2B; TF2B; TFIIB; TFIIB/GTF2B recombinant protein

• Host: E.coli
• Purity/Purification: > 85 % as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 um filtered solution of 20mM TrisHCl, 100mM NaCl, pH 8.0.
• Sequence Positions: Met1-Leu316

• Species: Human
• Endotoxin: < 1.0 EU per ug as determined by the LAL method.
• Tag: N-GST
• Reconstitution: We recommend that the vial be centrifuged to opening to bring the contents to the bottom. Do not mix by vortex. It is recommended that sterile water(50ul) be added to the vial to prepare a stock solution of 0.2mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Ships on blue ice. Lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

SDS-PAGE

Related Product Information for TFIIB/GTF2B recombinant protein

Transcription Initiation Factor IIB (TFIIB) is an essential factor for transcription by RNA Polymerase II. TFIIB localizes to the nucleus where it forms a complex (the DAB complex) with transcription factor IID and IIA. TFIIB plays a role as a bridge between IID, which initially recognizes the promoter sequence, and RNA polymerase II. TFIIB is involved in the selection of transcription start site.

NCBI and Uniprot Product Information

• NCBI GI #: 4504193
• NCBI GeneID: 2959
• NCBI Accession #: NP_001505.1
• NCBI GenBank Nucleotide #: NM_001514.5
• UniProt Accession #: Q00403; Q5JS30; A8K1A7
• Molecular Weight: Molecular Mass: 61.6 kDa; Actual Protein Molecular Mass: 62 kDa
• NCBI Official Full Name: transcription initiation factor IIB
• NCBI Official Synonym Full Names: general transcription factor IIB
• NCBI Official Symbol: GTF2B
• NCBI Official Synonym Symbols: TF2B; TFIIB
• NCBI Protein Information: transcription initiation factor IIB
• UniProt Protein Name: Transcription initiation factor IIB
• UniProt Gene Name: GTF2B
• UniProt Synonym Gene Names: TF2B; TFIIB

NCBI Description

This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq, Jul 2008]

Uniprot Description

General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA (PubMed:1876184, PubMed:1946368, PubMed:1517211, PubMed:3818643, PubMed:3029109, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:7601352, PubMed:9420329, PubMed:12931194, PubMed:27193682). Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex (PubMed:8504927, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312). Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle (PubMed:7601352). Associates with chromatin to core promoter-specific regions (PubMed:12931194, PubMed:24441171). Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element (PubMed:9420329, PubMed:16230532, PubMed:7675079, PubMed:10619841). Modulates transcription start site selection (PubMed:10318856). Exhibits also autoacetyltransferase activity that contributes to the activated transcription (PubMed:12931194).