PRSS2 cell lysate
Mouse PRSS2 HEK293 Overexpression Lysate
Gene Names
Prss2; Ta; TRY8; TRYP; Try2; Tesp4
Applications
Western Blot
Synonyms
PRSS2; Mouse PRSS2 HEK293 Overexpression Lysate; Mouse Trypsin 2/PRSS2 HEK293 Cell Lysate (WB positive control); protease; serine 2; Mouse Prss2 Overexpression Lysate; Mouse Ta Overexpression Lysate; Mouse Tesp4 Overexpression Lysate; Mouse Try2 Overexpression Lysate; Mouse TRY8 Overexpression Lysate; Mouse TRYP Overexpression Lysate; PRSS2 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
242aa
Sequence
Met1-Asn246
Applicable Applications for PRSS2 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse PRSS2 (NP_033456.1) precursor (Met 1-Asn 246) was expressed, with a C-terminal polyhistidine tag.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for PRSS2 cell lysate
Trypsin-2, also known as Trypsin II, Anionic trypsinogen, Serine protease 2, PRSS2 and TRY2, is a secreted protein that belongs to the trypsin serine protease family including Trypsin, PRSS1, PRSS2 and PRSS3. It consists of a signal peptide (residues 1-15), a pro region (residues 16-23), and a proteolytically active mature chain (residues 24-247). PRSS2 contains one peptidase S1 domain. It is secreted into the duodenum, hydrolysing peptides into their smaller building blocks, which is necessary for the uptake of protein in the food. It is secreted by the pancreas in the form of inactive zymogen, trypsinogen and cleaved to its active form in the small intestine when the pancreas is stimulated by cholecystokinin through the common activation mechanism.
This Mouse PRSS2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of PRSS2 protein from the overexpression lysate was verified.
This Mouse PRSS2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of PRSS2 protein from the overexpression lysate was verified.
Product Categories/Family for PRSS2 cell lysate
References
Rawlings, N.D. et al., 1994, Meth. Enzymol. 244: 19-61. Noone, P.G. et al., 2001, Gastroenterology. 121 (6): 1310-9. Leiros, H.K. et al., 2004, Protein Sci. 13 (4): 1056-70. Ronai, Z. et al., 2009, Biochem J. 418 (1):155-61.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
26,204 Da
NCBI Official Full Name
anionic trypsin-2
NCBI Official Synonym Full Names
protease, serine 2
NCBI Official Symbol
Prss2
NCBI Official Synonym Symbols
Ta; TRY8; TRYP; Try2; Tesp4
NCBI Protein Information
anionic trypsin-2
UniProt Protein Name
Anionic trypsin-2
Protein Family
UniProt Gene Name
Prss2
UniProt Synonym Gene Names
Try2
Uniprot Description
trypsin 2: In the ileum, may be involved in defensin processing, including DEFA5. Belongs to the peptidase S1 family.
Protein type: Calcium-binding; Cell adhesion; EC 3.4.21.4; Extracellular matrix; Protease; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 6|6 B1
Cellular Component: extracellular region; extracellular space
Molecular Function: calcium ion binding; hydrolase activity; metal ion binding; peptidase activity; serine-type endopeptidase activity; serine-type peptidase activity
Biological Process: collagen catabolic process; digestion; proteolysis; response to caffeine; response to nicotine
Research Articles on PRSS2
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Product Notes
The PRSS2 prss2 (Catalog #AAA8116572) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 242aa. AAA Biotech's PRSS2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the PRSS2 prss2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Asn24 6. It is sometimes possible for the material contained within the vial of "PRSS2, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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