NGFR active protein

NGFR Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8121315

• Gene Names: NGFR; CD271; p75NTR; TNFRSF16; p75(NTR); Gp80-LNGFR
• Purity: >95% as determined by SDS-PAGE
• Synonyms: NGFR; NGFR Protein; Human; Recombinant (hFc Tag); Human NGFR/P75 Protein (Fc Tag); CD271 Protein; Gp80-LNGFR Protein; p75(NTR) Protein; p75NTR Protein; TNFRSF16 Protein; nerve growth factor receptor (TNFR superfamily; member 16); NGFR active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asn250

• Species: Human
• Activity: Measured by its ability to inhibit NGF-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.5-3ug/mL in the presence of 4ng/mL Recombinant Human NGF.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Lys 29
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human NGFR (P08138) extracellular domain (Met 1-Asn 250) was fused with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for NGFR active protein

Background: Nerve growth factor receptors (NGFRs) belong to a large growth factor receptor family. NGFR includes two types of receptors: high-affinity nerve growth factor receptor and low-affinity nerve growth factor receptor. The high-affinity nerve growth factor receptor is also referred to as the Trk family whose members are bound by some neurotrophins with high affinity. Nerve growth factor binds with TrkA after being released from target cells, the NGF/TrkA complex is subsequently trafficked back to the cell body. The Low-affinity nerve growth factor receptor also named p75 which binds with all kinds of neurotrophins with low affinity. All four kinds of neurotrophins, including Nerve growth factor, Brain-derived neurotrophic factor, Neurotrophin-3, and Neurotrophin-4 bind to the p75. Studies have proved that NGFR acts as a molecular signal switch that determines cell death or survival by three steps. First, pro-nerve growth factor (prNGF) triggers cell apoptosis by its high-affinity binding to p75NTR, while NGF induces neuronal survival with low-affinity binding. Second, p75NTR mediates cell death by combining with co-receptor Sortilin, whereas it promotes neuronal survival through combination with proNGF. Third, the release of the intracellular domain chopper or cleavage short p75 NTR can independently initiate neuronal apoptosis.

Product Categories/Family for NGFR active protein

Death Receptor Signaling

References

Chen LW, et al. (2008) The proNGF-p75NTR-sortilin signalling complex as new target for the therapeutic treatment of Parkinson's disease. CNS Neurol Disord Drug Targets. 7(6): 512-23.Deponti D, et al. (2009) The low-affinity receptor for neurotrophins p75NTR plays a key role for satellite cell function in muscle repair acting via RhoA. Mol Biol Cell.20(16): 3620-7.Ken-ichiro K, et al. (2004) Necdin-related MAGE proteins differentially interact with the E2F1 transcription factor and the p75 neurotrophin receptor. J Biol Chem. 279 (3): 1703-12.

NCBI and Uniprot Product Information

• NCBI GI #: 4505393
• NCBI GeneID: 4804
• NCBI Accession #: NP_002498.1
• NCBI GenBank Nucleotide #: NM_002507.3
• UniProt Accession #: P08138; B2R961
• Molecular Weight: 45,183 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 16
• NCBI Official Synonym Full Names: nerve growth factor receptor
• NCBI Official Symbol: NGFR
• NCBI Official Synonym Symbols: CD271; p75NTR; TNFRSF16; p75(NTR); Gp80-LNGFR
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 16; p75 ICD; NGF receptor; TNFR superfamily, member 16; low affinity nerve growth factor receptor; low affinity neurotrophin receptor p75NTR; low-affinity nerve growth factor receptor
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 16
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: NGFR
• UniProt Synonym Gene Names: TNFRSF16; NGF receptor
• UniProt Entry Name: TNR16_HUMAN

NCBI Description

Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. [provided by RefSeq, Jul 2008]

Uniprot Description

NGFR: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Homodimer; disulfide-linked. Interacts with p75NTR- associated cell death executor. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1 and NGFRAP1/BEX3. Interacts with KIDINS220 and NTRK1. Can form a ternary complex with NTRK1 and KIDINS220 and this complex is affected by the expression levels of KIDINS220. An increase in KIDINS220 expression leads to a decreased association of NGFR and NTRK1. Interacts with NTRK2; may regulate the ligand specificity of the NTRK2 receptor. Interacts with LINGO1.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 17q21-q22

Cellular Component: nucleoplasm; cell surface; integral to plasma membrane; extracellular region; plasma membrane; cytosol; endosome

Molecular Function: signal transducer activity; protein binding; transmembrane receptor activity; ubiquitin protein ligase binding; death receptor activity; nerve growth factor binding; receptor activity; Rab GTPase binding

Biological Process: detection of temperature stimulus; axon guidance; hair follicle morphogenesis; membrane protein intracellular domain proteolysis; central nervous system development; nerve growth factor receptor signaling pathway; negative regulation of fibroblast growth factor receptor signaling pathway; positive regulation of apoptosis; positive regulation of odontogenesis of dentine-containing teeth; regulation of caspase activity; glucose homeostasis; regulation of axonogenesis; negative regulation of hair follicle development; negative regulation of cell cycle; intracellular protein transport; positive regulation of fibroblast proliferation; negative regulation of axonogenesis; positive regulation of axonogenesis; nerve development; circadian regulation of gene expression; negative regulation of apoptosis