Rabbit NGFR Polyclonal Antibody | anti-NGFR antibody

Anti-NGFR Antibody, Rabbit Polyclonal

Cat. Num. AAA8101645

• Gene Names: Ngfr; p75; LNGFR; p75NTR; RNNGFRR; Tnfrsf16
• Reactivity: Rat; Predicted to React with: Human, Mouse (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: NGFR; Polyclonal Antibody; Anti-NGFR Antibody; Rabbit Polyclonal; NGFR/P75 Antibody; Rabbit PAb; Antigen Affinity Purified; nerve growth factor receptor (TNFR superfamily; member 16); anti-NGFR antibody

• Host: Rabbit
• Reactivity: Rat; Predicted to React with: Human, Mouse (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rat NGFR
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-NGFR antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; ICC/IF: 1:300-1:10,000
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Rat NGFR/P75
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Rat NGFR/P75, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-NGFR rabbit polyclonal antibody at 1:500 dilution Lane A: ACHN Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:45 kDa Observed band size:45 kDa (We are unsure as to the identity of these extra bands.))

Immunofluorescence (IF)

(Immunofluorescence staining of NGFR in HESS9 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-rat NGFR polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cell membrane and cytoplasm.)

Related Product Information for anti-NGFR antibody

Nerve growth factor receptors (NGFRs) belong to a large growth factor receptor family. NGFR includes two types of receptors: high-affinity nerve growth factor receptor and low-affinity nerve growth factor receptor. The high-affinity nerve growth factor receptor is also referred to as the Trk family whose members are bound by some neurotrophins with high affinity. Nerve growth factor binds with TrkA after being released from target cells, the NGF/TrkA complex is subsequently trafficked back to the cell body. The Low-affinity nerve growth factor receptor also named p75 which binds with all kinds of neurotrophins with low affinity. All four kinds of neurotrophins, including Nerve growth factor, Brain-derived neurotrophic factor, Neurotrophin-3, and Neurotrophin-4 bind to the p75. Studies have proved that NGFR acts as a molecular signal switch that determines cell death or survival by three steps. First, pro-nerve growth factor (prNGF) triggers cell apoptosis by its high-affinity binding to p75NTR, while NGF induces neuronal survival with low-affinity binding. Second, p75NTR mediates cell death by combining with co-receptor Sortilin, whereas it promotes neuronal survival through combination with proNGF. Third, the release of the intracellular domain chopper or cleavage short p75 NTR can independently initiate neuronal apoptosis.

Product Categories/Family for anti-NGFR antibody

Death Receptor Signaling

References

Chen LW, et al. (2008) The proNGF-p75NTR-sortilin signalling complex as new target for the therapeutic treatment of Parkinson's disease. CNS Neurol Disord Drug Targets. 7(6): 512-23.Deponti D, et al. (2009) The low-affinity receptor for neurotrophins p75NTR plays a key role for satellite cell function in muscle repair acting via RhoA. Mol Biol Cell.20(16): 3620-7.Ken-ichiro K, et al. (2004) Necdin-related MAGE proteins differentially interact with the E2F1 transcription factor and the p75 neurotrophin receptor. J Biol Chem. 279 (3): 1703-12.

NCBI and Uniprot Product Information

• NCBI GI #: 291490673
• NCBI GeneID: 24596
• NCBI Accession #: NP_036742.2
• NCBI GenBank Nucleotide #: NM_012610.2
• UniProt Accession #: P07174
• Molecular Weight: 45,433 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 16
• NCBI Official Synonym Full Names: nerve growth factor receptor
• NCBI Official Symbol: Ngfr
• NCBI Official Synonym Symbols: p75; LNGFR; p75NTR; RNNGFRR; Tnfrsf16
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 16; p75 ICD; gp80-LNGFR; NGF receptor; nerve growth factor receptor, fast; low affinity nerve growth factor receptor; low affinity neurotrophin receptor p75NTR; low-affinity nerve growth factor receptor; n
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 16
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: Ngfr
• UniProt Synonym Gene Names: Tnfrsf16; NGF receptor
• UniProt Entry Name: TNR16_RAT

NCBI Description

receptor that can bind NGF, BDNF, NT-3, and NT-4 [RGD, Feb 2006]

Uniprot Description

Function: Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake. Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Necessary for the circadian oscillation of the clock genes ARNTL/BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver.1 PublicationManual assertion based on experiment in:Ref.4