Rabbit anti-Human NR0B1 Polyclonal Antibody | anti-NR0B1 antibody

NR0B1 Antibody

Cat. Num. AAA7052197

• Gene Names: NR0B1; AHC; AHX; DSS; GTD; HHG; AHCH; DAX1; DAX-1; NROB1; SRXY2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >95%, Protein G purified
• Synonyms: NR0B1; Polyclonal Antibody; NR0B1 Antibody; Nuclear receptor subfamily 0 group B member 1; DSS-AHC critical region on the X chromosome protein 1; Nuclear receptor DAX-1; AHC; DAX1; anti-NR0B1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 470

• Applicable Applications for anti-NR0B1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:5000; IHC: 1:20-1:200
• Immunogen: Recombinant Human Nuclear receptor subfamily 0 group B member 1 protein (1-243AA)
• Conjugation: Non-conjugated
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: MCF-7 whole cell lysate,Hela whole cell lysateAll lanes: NR0B1 antibody at 2.8ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 52,44 KDaObserved band size: 52 KDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human testis tissue using MBS7052197 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human small intestine tissue using MBS7052197 at dilution of 1:100)

Related Product Information for anti-NR0B1 antibody

Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 5016090
• NCBI GeneID: 190
• NCBI Accession #: NP_000466.2
• NCBI GenBank Nucleotide #: NM_000475.4
• UniProt Accession #: P51843; Q96F69
• Molecular Weight: 43,590 Da
• NCBI Official Full Name: nuclear receptor subfamily 0 group B member 1
• NCBI Official Synonym Full Names: nuclear receptor subfamily 0 group B member 1
• NCBI Official Symbol: NR0B1
• NCBI Official Synonym Symbols: AHC; AHX; DSS; GTD; HHG; AHCH; DAX1; DAX-1; NROB1; SRXY2
• NCBI Protein Information: nuclear receptor subfamily 0 group B member 1
• UniProt Protein Name: Nuclear receptor subfamily 0 group B member 1
• Protein Family: Nuclear receptor subfamily
• UniProt Gene Name: NR0B1
• UniProt Synonym Gene Names: AHC; DAX1

NCBI Description

This gene encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in this gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism. [provided by RefSeq, Jul 2008]

Uniprot Description

NR0B1: orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency. Homodimerizes with STF-1, NR5A2, NR0B2 and with COPS2. Shuttles between the cytoplasm and nucleus. Homodimers exits in the cytoplasm and in the nucleus. Homodimerization involved an interaction between amino and carboxy termini involving LXXLL motifs and steroid binding domain (AF-2 motif). Heterodimerizes with STF-1 and NROB2 through its N-terminal LXXLL motifs. Defects in NR0B1 are the cause of congenital X-linked adrenal hypoplasia. Two alternatively spliced human isoforms have been described.

Protein type: Nuclear receptor

Chromosomal Location of Human Ortholog: Xp21.2

Cellular Component: cytoplasm; membrane; nucleoplasm; nucleus; polysomal ribosome

Molecular Function: DNA binding; DNA hairpin binding; ligand-dependent nuclear receptor activity; protein binding; protein domain specific binding; protein homodimerization activity; RNA binding; sequence-specific DNA binding; steroid hormone receptor binding; transcription corepressor activity; transcription factor binding

Biological Process: adrenal gland development; gonad development; male gonad development; negative regulation of steroid hormone receptor signaling pathway; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; protein localization; steroid biosynthetic process; transcription initiation from RNA polymerase II promoter

Disease: 46,xy Sex Reversal 2; Adrenal Hypoplasia, Congenital