HER4 / ErbB4 active protein

Recombinant Rat HER4 / ErbB4 Protein (His tag)

Cat. Num. AAA2547456

• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: HER4 / ErbB4; Recombinant Rat HER4 / ErbB4 Protein (His tag); ERBB4; HER4 / ErbB4 active protein

• Host: HEK293 cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4, 5% trehalose, 5% mannitol, 0.01% Tween 80
• Sequence Length: 1292

• Application Notes: The recombinant rat ERBB4 comprises 637 amino acids and predicts a molecular mass of 71.3 kDa. The apparent molecular mass of the recombinant protein is approximately 75-95 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Predicted N Terminal: Gln 26
• Source: Rat
• Bio-Activity: Measured by its bingding ability in a functional ELISA. Immobilized rat ERBB4-His at 10 ug/mL (100 uL/well) can bind biotinylated human NRG1, the EC50 of biotinylated human NRG1 is 0.68-1.6 ug /mL.
• Endotoxin: < 1.0 EU per ug of the protein as determined by the LAL method.
• Reconstitution: It is recommended that sterile water (200 ul) be added to the vial to prepare a stock solution of 0.25 mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted and be used as soon as possible. Avoid repeated freeze-thaw cycles.

SDS-PAGE

Related Product Information for HER4 / ErbB4 active protein

Background: ERBB4 is a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. ERBB4 is expressed at highest levels in brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis and breast. And lower levels in thymus, lung, salivary gland, and pancreas. It specifically binds to and is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. ERBB4 also can be activated by other factors and induces a variety of cellular responses including mitogenesis and differentiation. ERBB4 regulates development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. It is required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. ERBB4 also play a role on the normal development of the embryonic central nervous system, especially for normal neural crest cell migration and normal axon guidance. It is required for mammary gland differentiation, induction of milk proteins and lactation.

Description: A DNA sequence encoding the rat ERBB4 (AAQ77349.1) (Met1-Pro651) was expressed with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 34597585
• NCBI GeneID: 59323
• Molecular Weight: The recombinant rat ERBB4 comprises 637 amino acids and predicts a molecular mass of 71.3 kDa. The apparent molecular mass of the recombinant protein is approximately 75-95 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• NCBI Official Full Name: receptor tyrosine kinase isoform JMa cyt2
• NCBI Official Synonym Full Names: erb-b2 receptor tyrosine kinase 4
• NCBI Official Symbol: Erbb4
• NCBI Protein Information: receptor tyrosine-protein kinase erbB-4
• UniProt Protein Name: Receptor tyrosine-protein kinase erbB-4
• UniProt Gene Name: Erbb4
• UniProt Synonym Gene Names: Tyro-2; 4ICD; E4ICD

NCBI Description

binds neuregulins; involved in epithelial-mesenchymal interactions during development; may play a role in tooth morphogenesis [RGD, Feb 2006]

Uniprot Description

HER4: a receptor tyrosine kinase of the EGFR family. Specifically binds and is activated by neuregulins, NRG- 2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. Heterodimerizes and signals with other EGF receptors. Interaction with these factors induces cell differentiation. Not activated by EGF, TGF-A, and amphiregulin. Interacts with PDZ domains of DLG2, DLG3, DLG4 and the syntrophin SNTB2. Interacts with WWOX. May act as a tumor suppressor: overexpressed in head and neck cancer , but downregulated in renal cancer, papillary carcinoma, high-grade gliomas and invasive breast cancer. 3 alternatively-spliced isoforms of the human protein have been reported.

Protein type: EC 2.7.10.1; EGFR family; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 9q32-q33

Cellular Component: basolateral plasma membrane; caveola; cytoplasm; lipid raft; mitochondrion; nucleus; plasma membrane; postsynaptic density; receptor complex

Molecular Function: epidermal growth factor receptor binding; protein binding; protein homodimerization activity; protein-tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity

Biological Process: cell fate commitment; cell migration; central nervous system morphogenesis; embryonic pattern specification; heart development; lactation; mammary gland development; mitochondrial fragmentation during apoptosis; negative regulation of apoptosis; negative regulation of cell proliferation; nervous system development; neural crest cell migration; odontogenesis; olfactory bulb interneuron differentiation; peptidyl-tyrosine phosphorylation; positive regulation of apoptosis; positive regulation of cardiac muscle cell proliferation; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of epithelial cell proliferation; positive regulation of glucose import; positive regulation of phosphoinositide 3-kinase activity; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of synaptic transmission, GABAergic; positive regulation of transcription, DNA-dependent; positive regulation of tyrosine phosphorylation of Stat5 protein; protein amino acid autophosphorylation; regulation of cell migration; response to drug; response to hydrogen peroxide; response to progesterone stimulus; signal transduction; surfactant homeostasis; synapse maturation; telencephalon development; transmembrane receptor protein tyrosine kinase signaling pathway