ERBB4 active protein

ErbB4 Protein, Rat, Recombinant (hFc Tag)

Cat. Num. AAA8123358

• Purity: >95% as determined by SDS-PAGE
• Synonyms: ErbB4; ErbB4 Protein; Rat; Recombinant (hFc Tag); Rat HER4/ErbB4 Protein (Fc Tag); erb-b2 receptor tyrosine kinase 4; ERBB4 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Pro651

• Species: Rat
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 26
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the rat ERBB4 (AAQ77349.1)(Met1-Pro651) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its binding ability in a functional ELISA. Immobilized rat ERBB4-Fc at 10ug/ml (100uL/well) can bind biotinylated human NRG1. The EC50 of biotinylated human NRG1 is 0.69-1.61ug/ml.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for ERBB4 active protein

Background: ERBB4 is a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. ERBB4 is expressed at highest levels in brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis and breast. And lower levels in thymus, lung, salivary gland, and pancreas. It specifically binds to and is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. ERBB4 also can be activated by other factors and induces a variety of cellular responses including mitogenesis and differentiation. ERBB4 regulates development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. It is required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. ERBB4 also play a role on the normal development of the embryonic central nervous system, especially for normal neural crest cell migration and normal axon guidance. It is required for mammary gland differentiation, induction of milk proteins and lactation.

Product Categories/Family for ERBB4 active protein

Cancer Drug Targets Receptor Tyrosine Kinases (RTKs)

References

Huang, Y Z, et al. (2000) Regulation of neuregulin signaling by PSD-95 interacting with ErbB4 at CNS synapses. Neuron. 26(2):443-55.
Garcia, R A, et al. (2000) The neuregulin receptor ErbB-4 interacts with PDZ-containing proteins at neuronal synapses. Proc Natl Acad Sci. 97(7):3596-601.
Silberberg G, et al. (2006) The involvement of ErbB4 with schizophrenia: association and expression studies. Am J Med Genet. 141(B2):142-8.
Sardi SP, et al. (2006) Presenilin-dependent ErbB4 nuclear signaling regulates the timing of astrogenesis in the developing brain. Cell. 127(1):185-97.

NCBI and Uniprot Product Information

• NCBI GI #: 34597585
• NCBI GeneID: 59323
• Molecular Weight: The recombinant rat ERBB4 comprises 637 amino acids and predicts a molecular mass of 71.3 kDa. The apparent molecular mass of the recombinant protein is approximately 75-95 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• NCBI Official Full Name: receptor tyrosine kinase isoform JMa cyt2
• NCBI Official Synonym Full Names: erb-b2 receptor tyrosine kinase 4
• NCBI Official Symbol: Erbb4
• NCBI Protein Information: receptor tyrosine-protein kinase erbB-4
• Protein Family: Receptor tyrosine-protein kinase

NCBI Description

binds neuregulins; involved in epithelial-mesenchymal interactions during development; may play a role in tooth morphogenesis [RGD, Feb 2006]