HER4 / ErbB4 recombinant protein

Recombinant Mouse HER4 / ErbB4 Protein (His tag)

Cat. Num. AAA2546677

• Gene Names: Gpnmb; ipd; Dchil; DC-HIL
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: HER4 / ErbB4; Recombinant Mouse HER4 / ErbB4 Protein (His tag); ERBB4; Mer4; HER4 / ErbB4 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 574

• Application Notes: The recombinant mouse ERBB4 comprises 638 amino acids and has a predicted molecular mass of 71.5 kDa. The apparent molecular mass of the protein is approximately 113 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Predicted N Terminal: Gln 26
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for HER4 / ErbB4 recombinant protein

Background: ERBB4 is a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. ERBB4 is expressed at highest levels in brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis and breast. And lower levels in thymus, lung, salivary gland, and pancreas. It specifically binds to and is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. ERBB4 also can be activated by other factors and induces a variety of cellular responses including mitogenesis and differentiation. ERBB4 regulates development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. It is required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. ERBB4 also play a role on the normal development of the embryonic central nervous system, especially for normal neural crest cell migration and normal axon guidance. It is required for mammary gland differentiation, induction of milk proteins and lactation.

Description: A DNA sequence encoding the mouse ERBB4 (Q99P91) (Met1-Leu652) was expressed with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 171846253
• NCBI GeneID: 93695
• NCBI Accession #: NP_444340.3
• NCBI GenBank Nucleotide #: NM_053110.4
• UniProt Accession #: Q99P91; Q3U3R9; Q8BVV9; Q8BXL4; Q9QXA0
• Molecular Weight: 63,676 Da
• NCBI Official Full Name: transmembrane glycoprotein NMB
• NCBI Official Synonym Full Names: glycoprotein (transmembrane) nmb
• NCBI Official Symbol: Gpnmb
• NCBI Official Synonym Symbols: ipd; Dchil; DC-HIL
• NCBI Protein Information: transmembrane glycoprotein NMB
• UniProt Protein Name: Transmembrane glycoprotein NMB
• UniProt Gene Name: Gpnmb
• UniProt Synonym Gene Names: Dchil; Hgfin; Nmb

Uniprot Description

GPNMB: Could be a melanogenic enzyme. Belongs to the PMEL/NMB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6 B2.3|6 23.82 cM

Cellular Component: cytoplasmic vesicle; integral to membrane; integral to plasma membrane

Molecular Function: heparin binding; integrin binding; syndecan binding

Biological Process: cell adhesion; negative regulation of cytokine production; negative regulation of T cell proliferation; negative regulation of tumor necrosis factor production; positive regulation of cell migration; positive regulation of protein amino acid autophosphorylation; positive regulation of protein amino acid phosphorylation; regulation of tissue remodeling