Human nerve growth factor (beta polypeptide) ELISA Kit | NGF elisa kit

Human Nerve growth factor, NGF ELISA Kit

Cat. Num. AAA914982

• Gene Names: NGF; NGFB; HSAN5; Beta-NGF
• Reactivity: Human
• Synonyms: nerve growth factor (beta polypeptide); Human Nerve growth factor; NGF ELISA Kit; NGF ELISA kit; Beta-NGF; HSAN5; MGC161426; MGC161428; NGFB; beta-nerve growth factor; nerve growth factor; beta polypeptide; beta subunit; NGF elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human NGF. No significant cross-reactivity or interference between human NGF and analogues was observed.
• Sequence Length: 241

• Samples: Serum, plasma, cell culture supernates, tissue homogenates, cell lysates
• Assay Type: Quantitative Sandwich
• Detection Range: 6.8 pg/ml-5000 pg/ml
• Sensitivity: <6.8 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NGF elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NGF bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 70995319
• NCBI GeneID: 4803
• NCBI Accession #: NP_002497.2
• NCBI GenBank Nucleotide #: NM_002506.2
• UniProt Accession #: P01138; Q6FHA0; Q96P60; Q9P2Q8; Q9UKL8; A1A4E5
• Molecular Weight: 26,959 Da
• NCBI Official Full Name: beta-nerve growth factor
• NCBI Official Synonym Full Names: nerve growth factor (beta polypeptide)
• NCBI Official Symbol: NGF
• NCBI Official Synonym Symbols: NGFB; HSAN5; Beta-NGF
• NCBI Protein Information: beta-nerve growth factor; nerve growth factor, beta subunit
• UniProt Protein Name: Beta-nerve growth factor
• Protein Family: Nerve growth factor
• UniProt Gene Name: NGF
• UniProt Synonym Gene Names: NGFB; Beta-NGF
• UniProt Entry Name: NGF_HUMAN

NCBI Description

This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq, Jul 2008]

Uniprot Description

NGF: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Homodimer. Belongs to the NGF-beta family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: extracellular space; Golgi lumen; cytoplasmic membrane-bound vesicle; extracellular region; endosome

Molecular Function: metalloendopeptidase inhibitor activity; protein binding; nerve growth factor receptor binding; growth factor activity; receptor signaling protein activity

Biological Process: circadian rhythm; response to nicotine; response to peptide hormone stimulus; nerve growth factor receptor signaling pathway; activation of MAPKK activity; positive regulation of apoptosis; adult locomotory behavior; regulation of neuron differentiation; positive regulation of axon extension; response to glucocorticoid stimulus; regulation of neurotransmitter secretion; response to lipopolysaccharide; regulation of caspase activity; sensory perception of pain; regulation of axonogenesis; negative regulation of cell cycle; nerve growth factor processing; response to radiation; cell-cell signaling; small GTPase mediated signal transduction; negative regulation of neuron apoptosis; response to electrical stimulus; inflammatory response; response to drug; positive regulation of neuron maturation; phosphoinositide-mediated signaling; positive regulation of protein amino acid autophosphorylation; memory; regulation of release of sequestered calcium ion into cytosol; positive regulation of nerve growth factor receptor signaling pathway; peripheral nervous system development; neuron apoptosis; induction of apoptosis via death domain receptors; response to mechanical stimulus; phospholipase C activation; response to ozone; Ras protein signal transduction; positive regulation of transcription factor activity; positive regulation of axonogenesis; neurite morphogenesis; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis

Disease: Neuropathy, Hereditary Sensory And Autonomic, Type V