Testing Data
GHR/Growth Hormone Receptor Active Protein | GHR active protein
Human CellExp GHR / Growth Hormone Receptor, human recombinant
Applications
SDS-Page
Purity
>=95%
Synonyms
GHR/Growth Hormone Receptor; Human CellExp GHR / Growth Hormone Receptor; human recombinant; GHR; GHBP; GH receptor; GHR active protein
Host
HEK293 cells
Purity/Purification
>=95%
Form/Format
Lyophilized from 0.22 mum filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Appearance: Lyophilized
Appearance: Lyophilized
Applicable Applications for GHR active protein
SDS-PAGE
Solubility/Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
Endotoxin Level
<1 EU/mug by LAL method
Biological Activity
The bioactivity is measured by its binding ability to human GH in a SPR assay. Covalently immobilized Recombinant Human GHR Protein can bind human growth hormone protein with affinity constant of 1.28 +/-0.16 nM range.
Results
The bioactivity is measured by its binding ability to human GH in a SPR assay. Covalently immobilized Recombinant Human GHR Protein can bind human growth hormone protein with affinity constant of 1.28 +/-0.16 nM range.
Handling
Centrifuge the vial prior to opening.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Testing Data
Testing Data
Related Product Information for GHR active protein
Background: Growth hormone receptor (GHR) is also known as somatotropin receptor, growth hormone-binding protein (GHBR), which belongs to the type I cytokine receptor family or Type 1 subfamily. GHR contains one fibronectin type-III domain. GHR / GHBR is expressed in various tissues with high expression in liver and skeletal muscle. The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. GHR is receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling.
Product Categories/Family for GHR active protein
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
This protein is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 28.5 kDa. The predicted N-terminus is Ala 27. DTT-reduced Protein migrates as 40-50 kDa in SDS-PAGE due to glycosylation.
Protein Family
Similar Products
Product Notes
The GHR (Catalog #AAA844185) is an Active Protein produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GHR/Growth Hormone Receptor can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the GHR for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GHR/Growth Hormone Receptor, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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