Rabbit anti-Human TNFAIP3 Polyclonal Antibody | anti-TNFAIP3 antibody

TNFAIP3, CT (Tumor Necrosis Factor alpha-induced Protein 3, TNF alpha-induced Protein 3, OTU Domain-containing Protein 7C, Putative DNA-binding Protein A20, Zinc Finger Protein A20, OTUD7C)

Cat. Num. AAA649891

• Gene Names: TNFAIP3; A20; OTUD7C; TNFA1P2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: TNFAIP3; Polyclonal Antibody; CT (Tumor Necrosis Factor alpha-induced Protein 3; TNF alpha-induced Protein 3; OTU Domain-containing Protein 7C; Putative DNA-binding Protein A20; Zinc Finger Protein A20; OTUD7C); Anti -TNFAIP3; anti-TNFAIP3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human TNFAIP3. Species sequence homology: mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-TNFAIP3 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1-2ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to 14aa from human TNFAIP3 at C-terminus.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(Western blot analysis of TNFAIP3 in SK-N-SH cell lysate with TNFAIP3 antibody at 1ug/ml.)

Related Product Information for anti-TNFAIP3 antibody

Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. In vitro able to deubiquitinate both 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system.

Product Categories/Family for anti-TNFAIP3 antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 395393997
• NCBI GeneID: 7128
• NCBI Accession #: NP_001257437.1
• NCBI GenBank Nucleotide #: NM_001270508.1
• UniProt Accession #: P21580; Q2HIX9; Q5VXQ7; Q9NSR6; B2R767; E1P588
• Molecular Weight: 89,614 Da
• NCBI Official Full Name: tumor necrosis factor alpha-induced protein 3
• NCBI Official Synonym Full Names: tumor necrosis factor, alpha-induced protein 3
• NCBI Official Symbol: TNFAIP3
• NCBI Official Synonym Symbols: A20; OTUD7C; TNFA1P2
• NCBI Protein Information: tumor necrosis factor alpha-induced protein 3; zinc finger protein A20; TNF alpha-induced protein 3; OTU domain-containing protein 7C; putative DNA-binding protein A20; tumor necrosis factor inducible protein A20
• UniProt Protein Name: Tumor necrosis factor alpha-induced protein 3
• Protein Family: TNFAIP3-interacting protein
• UniProt Gene Name: TNFAIP3
• UniProt Synonym Gene Names: OTUD7C; TNF alpha-induced protein 3
• UniProt Entry Name: TNAP3_HUMAN

NCBI Description

This gene was identified as a gene whose expression is rapidly induced by the tumor necrosis factor (TNF). The protein encoded by this gene is a zinc finger protein and ubiqitin-editing enzyme, and has been shown to inhibit NF-kappa B activation as well as TNF-mediated apoptosis. The encoded protein, which has both ubiquitin ligase and deubiquitinase activities, is involved in the cytokine-mediated immune and inflammatory responses. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

Function: Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. In cooperation with TAX1BP1 promotes disassembly of E2-E3 ubiquitin protein ligase complexes in IL-1R and TNFR-1 pathways; affected are at least E3 ligases TRAF6, TRAF2 and BIRC2, and E2 ubiquitin-conjugating enzymes UBE2N and UBE2D3. In cooperation with TAX1BP1 promotes ubiquitination of UBE2N and proteasomal degradation of UBE2N and UBE2D3. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Deubiquinates TRAF6 probably acting on 'Lys-63'-linked polyubiquitin. Upon T-cell receptor (TCR)-mediated T-cell activation, deubiquitinates 'Lys-63'-polyubiquitin chains on MALT1 thereby mediating disassociation of the CBM (CARD11:BCL10:MALT1) and IKK complexes and preventing sustained IKK activation. Deubiquinates NEMO/IKBKG; the function is facilitated by TNIP1 and leads to inhibition of NF-kappa-B activation. Upon stimulation by bacterial peptidoglycans, probably deubiquitinates RIPK2. Can also inhibit I-kappa-B-kinase (IKK) through a non-catalytic mechanism which involves polyubiquitin; polyubiquitin promotes association with IKBKG and prevents IKK MAP3K7-mediated phosphorylation. Targets TRAF2 for lysosomal degradation. In vitro able to deubiquitinate 'Lys-11'-, 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system. Required for LPS-induced production of proinflammatory cytokines and IFN beta in LPS-tolerized macrophages. Ref.8 Ref.9 Ref.10 Ref.13 Ref.14 Ref.16 Ref.19 Ref.21 Ref.24 Ref.25 Ref.27 Ref.28

Catalytic activity: Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). Ref.25 Ref.27 Ref.28

Subunit structure: Homodimer. Interacts with TNIP1, TAX1BP1 and TRAF2. Interacts with RNF11, ITCH and TAX1BP1 only after TNF stimulation; these interaction are transient and they are lost after 1 hour of stimulation with TNF

By similarity. Interacts with YWHAZ and YWHAH. Interacts with IKBKG; the interaction is induced by TNF stimulation and by polyubiquitin. Interacts with RIPK1. Interacts with UBE2N; the interaction requires TAX1BP1. Interacts with TRAF6; the interaction is inhibited by HTLV-1 protein Tax. Ref.8 Ref.9 Ref.10 Ref.11 Ref.16 Ref.20 Ref.24

Subcellular location: Cytoplasm Ref.12 Ref.19 Ref.23. Nucleus. Lysosome Ref.12 Ref.19 Ref.23. A20p50: Cytoplasm Ref.12 Ref.19 Ref.23.

Induction: By TNF.

Domain: The A20-type zinc fingers mediate the ubiquitin ligase activity. The A20-type zinc finger 4 selectively recognizes 'Lys-63'-linked polyubiquitin. The A20-type zinc finger 4-7 are sufficient to bind polyubiquitin. Ref.14The OTU domain mediates the deubiquitinase activity. Ref.14

Post-translational modification: Proteolytically cleaved by MALT1 upon TCR stimulation; disrupts NF-kappa-B inhibitory function and results in increased IL-2 production. It is proposed that only a fraction of TNFAIP3 colocalized with TCR and CBM complex is cleaved, leaving the main TNFAIP3 pool intact. Ref.17 Ref.23

Sequence similarities: Belongs to the peptidase C64 family.Contains 7 A20-type zinc fingers.Contains 1 OTU domain.