Decorin/Bone proteoglycan II Active Protein | DCN active protein

Human CellExp Decorin / Bone proteoglycan II, human recombinant

Cat. Num. AAA845206

• Gene Names: DCN; CSCD; PG40; PGII; PGS2; DSPG2; SLRR1B
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: Decorin/Bone proteoglycan II; Human CellExp Decorin / Bone proteoglycan II; human recombinant; Decorin; DCN; CSCD; DSPG2; PG40; PGII; PGS2; SLRR1B.; DCN active protein

• Host: HEK293 cells
• Purity/Purification: >=95%
• Form/Format: Lyophilized from 0.22 mum filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.; Appearance: Lyophilized
• Sequence Length: 359

• Applicable Applications for DCN active protein: SDS-PAGE
• Solubility/Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
• Endotoxin Level: <1 EU/mug by LAL method
• Biological Activity: Measured by its ability to modulate collagen fibrillogenesis. At 5 ug/mL, rh Decorin can significantly delay the rate of type I collagen fibrillogenesis.
• Results: Measured by its ability to modulate collagen fibrillogenesis. At 5 ug/mL, rh Decorin can significantly delay the rate of type I collagen fibrillogenesis.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for DCN active protein

Background: Decorin, also known as bone proteoglycan II, PGS2, SLRR1B, DCN, DSPG2 and PG40, is a secreted chondroitin /dermatan sulfate proteoglycan in the family of small leucine-rich proteoglycans (SLRPs). Decorin is a small cellular or pericellular matrix proteoglycan and is closely related in structure to biglycan protein. Decorin and biglycan are thought to be the result of gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly. Decorin appears to influence fibrillogenesis, and also interacts with fibronectin, thrombospondin, the complement component C1q, epidermal growth factor receptor (EGFR) and transforming growth factor-beta (TGF-beta). Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD).

Product Categories/Family for DCN active protein

Proteins and Enzymes; Human cell expressed recombinant proteinsCytokines; Growth Factors & Hormones; Human cell expressed recombinant proteinsProteins and Enzymes; Cytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 4503271
• NCBI GeneID: 1634
• NCBI Accession #: NP_001911.1
• NCBI GenBank Nucleotide #: NM_001920.3
• UniProt Accession #: P07585; Q9P0Z0; Q9P0Z1; Q9Y5N8; Q9Y5N9
• Molecular Weight: This protein fused with 6×His tag at the C-terminus, has a calculated MW of 38.8 kDa. The predicted N-terminus is Gly 17. DTT-reduced Protein migrates as 45 kDa or higher due to different glycosylation.
• NCBI Official Full Name: decorin isoform a preproprotein
• NCBI Official Synonym Full Names: decorin
• NCBI Official Symbol: DCN
• NCBI Official Synonym Symbols: CSCD; PG40; PGII; PGS2; DSPG2; SLRR1B
• NCBI Protein Information: decorin
• UniProt Protein Name: Decorin
• Protein Family: Decorin
• UniProt Gene Name: DCN
• UniProt Synonym Gene Names: SLRR1B
• UniProt Entry Name: PGS2_HUMAN

NCBI Description

The protein encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to biglycan protein. The encoded protein and biglycan are thought to be the result of a gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly. It contains one attached glycosaminoglycan chain. This protein is capable of suppressing the growth of various tumor cell lines. There are multiple alternatively spliced transcript variants known for this gene. This gene is a candidate gene for Marfan syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

DCN: May affect the rate of fibrils formation. Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD). Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance. Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Extracellular matrix; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q21.33

Cellular Component: extracellular matrix; lysosomal lumen; extracellular space; proteinaceous extracellular matrix; Golgi lumen; extracellular region; collagen type VI

Molecular Function: collagen binding; glycosaminoglycan binding; extracellular matrix binding; protein N-terminus binding

Biological Process: extracellular matrix organization and biogenesis; skeletal muscle development; chondroitin sulfate biosynthetic process; wound healing; glycosaminoglycan metabolic process; pathogenesis; response to lipopolysaccharide; dermatan sulfate biosynthetic process; chondroitin sulfate metabolic process; extracellular matrix disassembly; organ morphogenesis; response to mechanical stimulus; chondroitin sulfate catabolic process; carbohydrate metabolic process; kidney development; peptide cross-linking via chondroitin 4-sulfate glycosaminoglycan; aging; placenta development

Disease: Corneal Dystrophy, Congenital Stromal