CD147 Active Protein | BSG active protein

Human CellExp CD147, human recombinant

Cat. Num. AAA844717

• Gene Names: BSG; M6; OK; 5F7; TCSF; CD147; EMMPRIN
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: CD147; Human CellExp CD147; human recombinant; Basigin; BSG; 5F7; EMMPRIN; M6; OK; TCSF.; BSG active protein

• Host: HEK293 cells
• Purity/Purification: >=95%
• Form/Format: Lyophilized from 0.22 mum filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.; Appearance: Lyophilized
• Sequence Length: 385

• Applicable Applications for BSG active protein: SDS-PAGE
• Solubility/Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
• Endotoxin Level: <1 EU/mug by LAL method
• Biological Activity: Measured by the ability of the immobilized protein to induce active MMP1 secretion by NHLF human normal lung fibroblasts. The ED50 for this effect is typically 0.5-4 mug/ml.
• Results: Measured by the ability of the immobilized protein to induce active MMP1 secretion by NHLF human normal lung fibroblasts. The ED50 for this effect is typically 0.5-4 mug/ml.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for BSG active protein

Background: CD147, also known as Basigin (BSG), or extracellular matrix metalloproteinase inducer (EMMPRIN). The human basigin protein contains 269 amino acids that form two heavily glycosylated C2 type immunoglobulin-like domains at the N-terminal extracellular portion. A second form of basigin has also been characterized that contains one additional immunoglobulin-like domain in its extracellular portion. As members of the immunoglobulin superfamily play fundamental roles in intercellular recognition involved in various immunologic phenomena, differentiation, and development, basigin is thought also to play a role in intercellular recognition and regulate several distinct functions, such as spermatogenesis, expression of the monocarboxylate transporter and the responsiveness of lymphocytes. Basigin is a type I integral membrane receptor that has many ligands, including the cyclophilin (CyP) proteins Cyp-A and CyP-B and certain integrins.

Product Categories/Family for BSG active protein

Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteins Proteins and Enzymes; Human cell expressed recombinant proteinsProteins and Enzymes; Cytokines; Growth Factors & Hormones; CDsProteins and Enzymes; Cytokines; Growth Factors &

NCBI and Uniprot Product Information

• NCBI GI #: 38372919
• NCBI GeneID: 682
• NCBI Accession #: NP_001719.2
• NCBI GenBank Nucleotide #: NM_001728.3
• UniProt Accession #: P35613; Q7Z796; Q8IZL7; A6NJW1; D3YLG5
• Molecular Weight: This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 21.6 kDa. The predicted N-terminus is Ala 22. DTT-reduced Protein migrates as 26-33 kDa due to glycosylation.
• NCBI Official Full Name: basigin isoform 1
• NCBI Official Synonym Full Names: basigin (Ok blood group)
• NCBI Official Symbol: BSG
• NCBI Official Synonym Symbols: M6; OK; 5F7; TCSF; CD147; EMMPRIN
• NCBI Protein Information: basigin
• UniProt Protein Name: Basigin
• Protein Family: Basigin
• UniProt Gene Name: BSG
• UniProt Synonym Gene Names: EMMPRIN; TCSF
• UniProt Entry Name: BASI_HUMAN

NCBI Description

The protein encoded by this gene is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

BSG: Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. Enriched on the surface of tumor cells. Up-regulated in gliomas. Its expression levels correlate with malignant potential of the tumor. Forms homooligomers in a cis-dependent manner on the plasma membrane. Forms a complex with MMP1 at the tumor cell surface. Interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. Interacts with ATP1B2, MAG and L1CAM. Interacts with AJAP1. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: Golgi membrane; focal adhesion; membrane; mitochondrion; integral to plasma membrane; plasma membrane; melanosome; sarcolemma; acrosomal membrane; lipid raft

Molecular Function: mannose binding; protein binding

Biological Process: response to mercury ion; cellular metabolic process; response to peptide hormone stimulus; extracellular matrix disassembly; extracellular matrix organization and biogenesis; cell surface receptor linked signal transduction; response to cAMP; decidualization; pyruvate metabolic process; blood coagulation; leukocyte migration; embryo implantation; odontogenesis of dentine-containing teeth

Disease: Blood Group--ok