TNFRII active protein

Human CellExp TNFRII, human recombinant

Cat. Num. AAA845102

• Gene Names: TNFRSF1B; p75; TBPII; TNFBR; TNFR2; CD120b; TNFR1B; TNFR80; TNF-R75; p75TNFR; TNF-R-II
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: TNFRII; Human CellExp TNFRII; human recombinant; TNFRSF1B; CD120b; TNFRII active protein

• Host: HEK293 cells
• Purity/Purification: >=95%
• Form/Format: Lyophilized from 0.22 mum filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.; Appearance: Lyophilized
• Sequence Length: 461

• Applicable Applications for TNFRII active protein: SDS-PAGE
• Solubility/Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
• Endotoxin Level: <1 EU/mug by LAL method
• Biological Activity: Measured by its ability to inhibit the TNFalpha mediated cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.05-0.5 mug/mL in the presence of 0.25 ng/mL rhTNFalpha.
• Results: Measured by its ability to inhibit the TNFalpha mediated cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.05-0.5 mug/mL in the presence of 0.25 ng/mL rhTNFalpha.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for TNFRII active protein

Background: TNF RI (also known as the p60 or p55 TNFR) and TNF RII (the p75 or p80 TNFR) are two distinct type I transmembrane glycoproteins that bind TNF with high affinity. Both RI and RII are prototypic members of the TNF receptor superfamily and have been designated TNFRSF1A and TNFRSF1B, respectively. Human TNF RII cDNA encodes a 461 amino acid (aa) residue precursor protein with a 22 aa putative signal peptide, a 235 aa extracellular domain, a 20 aa transmembrane domain and a 174 aa cytoplasmic domain. TNFRII is expressed in fetal brain. The protein is produced naturally as a soluble form (sTNFRII). The soluble receptor inhibits TNFalpha action by competing with cell surface receptors in binding TNFalpha, thereby blocking its biologic effects. TNFRII is strongly expressed at the cartilage-pannus junction, and plays a major role in a subset of families with multiple cases of rheumatoid arthritis (RA). Further, high plasma levels of sTNFRII were significantly associated with increased incidence of coronary heart disease, independent of established cardiovascular risk factors, and seems to be useful for monitoring the inflammatory activity of sarcoidosis.

Product Categories/Family for TNFRII active protein

Proteins and Enzymes; Human cell expressed recombinant proteinsCytokines; Growth Factors & Hormones; Human cell expressed recombinant proteinsProteins and Enzymes; Cytokines; Growth Factors & Hormones; CDsProteins and Enzymes; Cytokines; Growth Factors &

NCBI and Uniprot Product Information

• NCBI GI #: 4507577
• NCBI GeneID: 7133
• NCBI Accession #: NP_001057.1
• NCBI GenBank Nucleotide #: NM_001066.2
• UniProt Accession #: P20333; Q16042; Q6YI29; Q9UIH1; B1AJZ3
• Molecular Weight: This protein is fused with a C-terminal 6×his tag, has a calculated MW of 26 kDa. The predicted N-terminus is Leu23. Protein migrates as 23-26 kDa in reduced SDS-PAGE due to glycosylation.
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 1B
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 1B
• NCBI Official Symbol: TNFRSF1B
• NCBI Official Synonym Symbols: p75; TBPII; TNFBR; TNFR2; CD120b; TNFR1B; TNFR80; TNF-R75; p75TNFR; TNF-R-II
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 1B
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 1B
• UniProt Gene Name: TNFRSF1B
• UniProt Synonym Gene Names: TNFBR; TNFR2; TNF-R2; TNF-RII; TNFR-II
• UniProt Entry Name: TNR1B_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. The function of IAPs in TNF-receptor signalling is unknown, however, c-IAP1 is thought to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. [provided by RefSeq, Jul 2008]

Uniprot Description

TNF-R2: Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity. Binds to TRAF2. Interacts with BMX. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 1p36.22

Cellular Component: cell soma; perinuclear region of cytoplasm; extracellular region; plasma membrane; integral to membrane; nucleus; lipid raft

Molecular Function: protein binding; tumor necrosis factor receptor activity; ubiquitin protein ligase binding

Biological Process: tumor necrosis factor-mediated signaling pathway; DNA damage response, signal transduction resulting in induction of apoptosis; negative regulation of inflammatory response; immune response; inflammatory response; RNA destabilization; positive regulation of membrane protein ectodomain proteolysis; aging