Neprilysin/CD10 Active Protein | MME active protein

Human CellExp Neprilysin / CD10, human recombinant

Cat. Num. AAA844735

• Gene Names: MME; NEP; SFE; CD10; CALLA
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: Neprilysin/CD10; Human CellExp Neprilysin / CD10; human recombinant; MME; CALLA; CD10; DKFZp686O16152; MGC126681; MGC126707; NEP; SFE; Neprilysin; MME active protein

• Host: HEK293 cells
• Purity/Purification: >=95%
• Form/Format: Lyophilized from 0.22 mum filtered solution in 50 mM tris, 150 mM NaCl, pH 8.0 Normally Mannitol or Trehalose is added as protectants before lyophilization.; Appearance: Lyophilized
• Sequence Length: 750

• Applicable Applications for MME active protein: SDS-PAGE
• Solubility/Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
• Endotoxin Level: <1 EU/mug by LAL method
• Biological Activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK-(Dnp)-OH. The specific activity is >2500 pmol/min/mug.
• Results: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK-(Dnp)-OH. The specific activity is >2500 pmol/min/mug.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for MME active protein

Background: Cluster of differentiation 10 (CD10), membrane metallo-endopeptidase, neutral endopeptidase (NEP), Neprilysin, and common acute lymphoblastic leukemia antigen (CALLA), is a 90-110-kDa type II transmembrane glycoprotein normally expressed by a variety of tissues, including epithelial cells of the prostate, kidney, intestine, endometrium, adrenal glands, and lung. This zinc-dependent metalloprotease enzyme cleaves peptide bonds on the amino side of hydrophobic residues and inactivates a variety of physiologically active secreted peptides. CD20 is thought to be the rate-limiting degrading enzyme of amyloid beta peptide (Abeta) whose abnormal misfolding and aggregation in neural tissue has been implicated in the development of Alzheimer's disease (AD). CD10 is also identified as the common acute lymphoblastic leukemia antigen (CALLA) present on leukemic cells of pre-B phenotype, and thus serves as the most important biomarker in the identification of human acute lymphocytic leukemia (ALL).

Product Categories/Family for MME active protein

Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteins Proteins and Enzymes; Human cell expressed recombinant proteinsProteins and Enzymes; Cytokines; Growth Factors & Hormones; CDsProteins and Enzymes; Cytokines; Growth Factors &

NCBI and Uniprot Product Information

• NCBI GI #: 116256329
• NCBI GeneID: 4311
• NCBI Accession #: NP_000893.2
• NCBI GenBank Nucleotide #: NM_000902.3
• UniProt Accession #: P08473; Q3MIX4; A8K6U6; D3DNJ9
• Molecular Weight: This protein with Gly-Pro at the N- terminus, has a calculated MW of 80 kDa. The predicted N-terminus is Tyr 52. DTT-reduced Protein migrates as 90-100 kDa due to glycosylation.
• NCBI Official Full Name: neprilysin
• NCBI Official Synonym Full Names: membrane metallo-endopeptidase
• NCBI Official Symbol: MME
• NCBI Official Synonym Symbols: NEP; SFE; CD10; CALLA
• NCBI Protein Information: neprilysin
• UniProt Protein Name: Neprilysin
• Protein Family: Neprilysin
• UniProt Gene Name: MME
• UniProt Synonym Gene Names: EPN; CALLA; NEP; Neutral endopeptidase; SFE
• UniProt Entry Name: NEP_HUMAN

NCBI Description

This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing. [provided by RefSeq, Jul 2008]

Uniprot Description

CD10: Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV- inducible elastase activity toward skin preelastic and elastic fibers. Inhibited in a dose dependent manner by opiorphin. Belongs to the peptidase M13 family.

Protein type: Protease; EC 3.4.24.11; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q25.2

Cellular Component: synaptic vesicle; focal adhesion; integral to plasma membrane; axon; cytoplasm; dendrite; plasma membrane; integral to membrane; synapse; brush border

Molecular Function: protein binding; zinc ion binding; metalloendopeptidase activity; endopeptidase activity; exopeptidase activity; peptide binding

Biological Process: cellular protein metabolic process; peptide metabolic process; beta-amyloid metabolic process; creatinine metabolic process; angiotensin maturation; sensory perception of pain; kidney development; proteolysis

Disease: Membranous Nephropathy, Susceptibility To