CD58 active protein

CD58 Protein, Cynomolgus, Recombinant (His Tag)

Cat. Num. AAA8123574

• Purity: >95% as determined by SDS-PAGE
• Synonyms: CD58; CD58 Protein; Cynomolgus; Recombinant (His Tag); Cynomolgus LFA-3/CD58 Protein (His Tag); LFA3 Protein; CD58 molecule; CD58 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Arg215

• Species: Cynomolgus
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ile 29
• Tag: C-His
• Protein Construction: A DNA sequence encoding the cynomolgus CD58 (F7HQR6)(Met1-Arg215) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus CD58-His at 10ug/ml (100uL/well) can bind Cynomolgus CD2-Fc. The EC50 of Cynomolgus CD2-Fc is 0.06-0.14ug/ml.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CD58 active protein

Background: CD53 is a member of the transmembrane 4 superfamily, also called the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. These proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD53 is a cell surface glycoprotein that is known to complex with integrins. Familial deficiency of CD53 gene has been linked to an immunodeficiency associated with recurrent infectious diseases caused by bacteria, fungi and viruses. CD53 contributes to the transduction of CD2-generated signals in T cells and natural killer cells and has been suggested to play a role in growth regulation.

References

Rochelle JM. et al., 1993, Int Immunol. 5 (2): 209-16. Virtaneva KI. et al., 1993, Immunogenetics. 37 (6): 461-5. Horejsa- V. et al., 1991, FEBS Lett. 288 (1-2): 1-4.

NCBI and Uniprot Product Information

• UniProt Accession #: F7HQR6
• Molecular Weight: The recombinant cynomolgus CD58 comprises 198 amino acids and has a calculated molecular mass of 23kDa. The apparent molecular mass of it is approximately 38-42kDa in SDS-PAGE under reducing conditions.
• Protein Family: Lymphocyte function-associated antigen