Rabbit Nhe-1 Polyclonal Antibody | anti-SLC9A1 antibody

Nhe-1 Antibody

Cat. Num. AAA150902

• Gene Names: SLC9A1; APNH; NHE1; NHE-1; PPP1R143
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Nhe-1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: Nhe-1; Polyclonal Antibody; Nhe-1 Antibody; APNH; NHE1; NHE-1; APNH1; solute carrier family 9 (sodium/hydrogen exchanger); member 1; anti-SLC9A1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Nhe-1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 815

• Applicable Applications for anti-SLC9A1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Nhe-1 antibody can be used for detection of Nhe-1 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: Nhe-1 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of the human Nhe-1.
• Buffer: Nhe-1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: Nhe-1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of Nhe-1 in rat kidney tissue lysate with in with Nhe-1 antibody at (A) 1 and (B) 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemical staining of human brain tissue using Nhe-1 antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of Nhe-1 in Human Brain tissue with Nhe-1 antibody at 20 μg/mL.)

Related Product Information for anti-SLC9A1 antibody

Nhe-1 Antibody: The Na+/H+ antiporter (Nhe-1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells and is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, and tumor promoters. Nhe-1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. This occurs through a previously unrecognized structural link between Nhe-1 and the actin-binding proteins ezrin, radixin, and moesin, collectively referred to as ERM proteins. A structural role for Nhe-1 has been proposed in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. It is also thought that Nhe-1 play a role in hypertension. At least two isoforms of Nhe-1 are known to exist.

NCBI and Uniprot Product Information

• NCBI GI #: 127809
• NCBI GeneID: 6548
• UniProt Accession #: P19634; Q96EM2; B1ALD6; D3DPL4
• Molecular Weight: 61,013 Da
• NCBI Official Full Name: Sodium/hydrogen exchanger 1
• NCBI Official Synonym Full Names: solute carrier family 9, subfamily A (NHE1, cation proton antiporter 1), member 1
• NCBI Official Symbol: SLC9A1
• NCBI Official Synonym Symbols: APNH; NHE1; NHE-1; PPP1R143
• NCBI Protein Information: sodium/hydrogen exchanger 1; Na(+)/H(+) exchanger 1; Na-Li countertransporter; protein phosphatase 1, regulatory subunit 143; solute carrier family 9 (sodium/hydrogen exchanger), member 1 (antiporter, Na+/H+, amiloride sensitive); solute carrier family 9 (sodium/hydrogen exchanger), isoform 1 (antiporter, Na+/H+, amiloride sensitive)
• UniProt Protein Name: Sodium/hydrogen exchanger 1
• Protein Family: Sodium/hydrogen exchanger
• UniProt Gene Name: SLC9A1
• UniProt Synonym Gene Names: APNH1; NHE1; NHE-1
• UniProt Entry Name: SL9A1_HUMAN

NCBI Description

This gene encodes a Na+/H+ antiporter that is a member of the solute carrier family 9. The encoded protein is a plasma membrane transporter that is expressed in the kidney and intestine. This protein plays a central role in regulating pH homeostasis, cell migration and cell volume. This protein may also be involved in tumor growth. [provided by RefSeq, Sep 2011]

Uniprot Description

NHE1: sodium/hydrogen exchanger 1. Belongs to the Na(+)/H(+) exchanger family. Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient.

Protein type: Transporter, SLC family; Membrane protein, multi-pass; Membrane protein, integral; Motility/polarity/chemotaxis; Transporter

Chromosomal Location of Human Ortholog: 1p36.1-p35

Cellular Component: endoplasmic reticulum membrane; focal adhesion; cell surface; mitochondrion; endoplasmic reticulum; basolateral plasma membrane; integral to plasma membrane; T-tubule; integral to membrane; lipid raft; nucleoplasm; perinuclear region of cytoplasm; lamellipodium; apical plasma membrane; cytoplasm; plasma membrane

Molecular Function: protein binding, bridging; calmodulin binding; phosphatidylinositol-4,5-bisphosphate binding; protein binding; protein complex scaffold; calcium-dependent protein binding; solute:hydrogen antiporter activity; sodium:hydrogen antiporter activity; protein phosphatase 2B binding

Biological Process: response to drug; cellular sodium ion homeostasis; cell migration; positive regulation of NFAT protein import into nucleus; glycosaminoglycan metabolic process; pathogenesis; maintenance of cell polarity; positive regulation of cell growth; cardiac muscle cell differentiation; protein oligomerization; hyaluronan catabolic process; cellular response to insulin stimulus; regulation of stress fiber formation; regulation of focal adhesion formation; regulation of intracellular pH; carbohydrate metabolic process; regulation of pH; ion transport; positive regulation of transcription from RNA polymerase II promoter; regulation of sensory perception of pain; response to acidity; positive regulation of action potential; cell growth; hyaluronan metabolic process; transmembrane transport; negative regulation of apoptosis

Disease: Lichtenstein-knorr Syndrome