Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human CD58 expression on human whole blood lymphocytes. Cells were stained with PE-conjugated anti-Human CD58. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.)
Mouse anti-Human CD58 Monoclonal Antibody | anti-CD58 antibody
Anti-CD58 Antibody (PE), Mouse Monoclonal
Gene Names
CD58; ag3; LFA3; LFA-3
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Protein A
Synonyms
CD58; Monoclonal Antibody; Anti-CD58 Antibody (PE); Mouse Monoclonal; LFA-3/CD58 Antibody (PE); Mouse MAb; CD58 molecule; Anti-ag3 Antibody; Anti-CD58 Antibody; Anti-LFA-3 Antibody; Anti-LFA3 Antibody; anti-CD58 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
05
Specificity
Human LFA-3/CD58
Purity/Purification
Protein A
Form/Format
Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Concentration
10ul/Test, 0.1mg/ml (varies by lot)
Applicable Applications for anti-CD58 antibody
Flow Cytometry (FC/FACS/FCM)
Immunogen
Recombinant Human LFA-3/CD58 Protein
Conjugation
PE
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human LFA-3/CD58 (rh LFA-3/CD58) and conjugated with PE under optimum conditions, the unreacted PE was removed.
Preparation and Storage
This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human CD58 expression on human whole blood lymphocytes. Cells were stained with PE-conjugated anti-Human CD58. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human CD58 expression on human whole blood lymphocytes. Cells were stained with PE-conjugated anti-Human CD58. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.)
Related Product Information for anti-CD58 antibody
CD53 is a member of the transmembrane 4 superfamily, also called the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. These proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD53 is a cell surface glycoprotein that is known to complex with integrins. Familial deficiency of CD53 gene has been linked to an immunodeficiency associated with recurrent infectious diseases caused by bacteria, fungi and viruses. CD53 contributes to the transduction of CD2-generated signals in T cells and natural killer cells and has been suggested to play a role in growth regulation.
References
Rochelle JM. et al., 1993, Int Immunol. 5 (2): 209-16. Virtaneva KI. et al., 1993, Immunogenetics. 37 (6): 461-5. HorejsiV. et al., 1991, FEBS Lett. 288 (1-2): 1-4.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
27,960 Da
NCBI Official Full Name
CD58 protein
NCBI Official Synonym Full Names
CD58 molecule
NCBI Official Symbol
CD58
NCBI Official Synonym Symbols
ag3; LFA3; LFA-3
NCBI Protein Information
lymphocyte function-associated antigen 3
Protein Family
NCBI Description
This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes. The protein is localized to the plasma membrane. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009]
Research Articles on CD58
Similar Products
Product Notes
The CD58 (Catalog #AAA8106404) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD58 Antibody (PE), Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD58 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS/FCM). Researchers should empirically determine the suitability of the CD58 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD58, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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