Western Blot (WB)
(Western blot analysis of SNF2H expression in PC3 (A), HepG2 (B), mouse spleen (C), mouse brain (D) whole cell lysates.)
Rabbit SNF2H Polyclonal Antibody | anti-SMARCA5 antibody
Anti-SNF2H Antibody
Western Blot (WB)
(Western blot analysis of SNF2H expression in PC3 (A), HepG2 (B), mouse spleen (C), mouse brain (D) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of SNF2H expression in PC3 (A), HepG2 (B), mouse spleen (C), mouse brain (D) whole cell lysates.)
Immunofluorescence (IF)
(Immunofluorescent analysis of SNF2H staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of SNF2H staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein. [provided by RefSeq, Jul 2008]
Uniprot Description
SMARCA5: an ubiquitous enzyme with ATP-dependent DNA helicase activity. Possesses intrinsic ATP-dependent nucleosome remodelling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin in vitro; this may require intact histone H4 tails. The catalytic subunit of the four known chromatin remodelling complexes: CHRAC, RSF, ACF/WCRF, and WICH. Each complex contains subunits which may regulate the specificity or catalytic activity of SMARCA5. Overexpressed in CD34-positive erythrocyte progenitor cells in acute myeloid leukemia. Down-regulation correlates with hematologic remission following chemotherapy. Belongs to the SNF2/RAD54 helicase family, ISWI subfamily.
Protein type: Helicase; EC 3.6.1.-; Transcription factor; EC 3.6.4.-; Histone-binding
Chromosomal Location of Human Ortholog: 4q31.1-q31.2
Cellular Component: chromatin silencing complex; condensed chromosome; nuclear replication fork; nucleolus; nucleoplasm; nucleus; RSF complex
Molecular Function: ATP binding; ATPase activity; DNA binding; helicase activity; histone binding; nucleosome binding; protein binding
Biological Process: ATP-dependent chromatin remodeling; chromatin remodeling; chromatin silencing at rDNA; DNA replication-independent nucleosome assembly at centromere; double-strand break repair; nucleosome assembly; nucleosome positioning; positive regulation of gene expression, epigenetic; positive regulation of transcription, DNA-dependent; regulation of transcription from RNA polymerase II promoter; transcription initiation
Research Articles on SMARCA5
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Product Notes
The SMARCA5 smarca5 (Catalog #AAA8245553) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SNF2H Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SNF2H can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP). WB: 1/500 - 1/2000; IF/ICC: 1/50 - 1/200; IP: 1/50 - 1/200; ChIP: 1/20 - 1/100. Researchers should empirically determine the suitability of the SMARCA5 smarca5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SNF2H, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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