CD28, Human/ Cynomolgus/Rhesus macaque Active Protein | CD28 active protein

Human CellExp CD28, Human/ Cynomolgus/Rhesus macaque Recombinant

Cat. Num. AAA8400094

• Gene Names: CD28; Tp44
• Purity: >90% by SDS-PAGE
• Synonyms: CD28; Human/ Cynomolgus/Rhesus macaque; Human CellExp CD28; Human/ Cynomolgus/Rhesus macaque Recombinant; T-cell-specific surface glycoprotein CD28; CD antigen: TP44; Tp44; T-Cell-Specific Surface Glycoprotein; CD28 Molecule; CD28 active protein

• Host: HEK293 cells
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Lyophilized powder
• Sequence: Asn19-Pro152

• Gene Source: Human/ Cynomolgus/Rhesus macaque
• Biological Activity: Human biotinylated CD28 can bind to immobilized human CD86 (at 5 ug/ml, 100 ul/well) with a linear detection range of 0.08 to 0.6 ug/ml.
• Reconstitution Instruction: Reconstitute in PBS, pH 7.4.
• Tag Note: This protein is fused with a polyhistidine tag at the C-terminus.The predicted N-terminus is Asn 19 (UniProtKB - P10747 CD28_HUMAN).
• Preparation and Storage: Store at -20 degree C; Centrifuge the vial prior to opening.; Shelf life: ~12 months

SDS-Page

Testing Data

Related Product Information for CD28 active protein

Human CD28 (Cluster of Differentiation 28) is expressed on the cell surface as a glycosylated, disulfide-linked homodimer. CD28 is the founding member of a subfamily of costimulatory molecules characterized by an extracellular variable immunoglobulin-like domain. Other members of the subfamily include ICOS, CTLA4, PD1, PD1H, and BTLA. CD28 and CTLA4 are highly homologous and compete for the same ligands B7-1 (CD80) and B7-2 (CD86). CD28 is expressed constitutively on almost all human CD4 T cells and approximately 50% of CD8 T cells. CD28 has diverse effects on T cell functions including the induction of cell proliferation and cytokine production and promotion of T-cell survival. Experiments showed that CD28 and CTLA4 have opposing effects on T cell stimulation. CD28 provides an activating signal and CTLA4 provides an inhibitory signal. Translation of a basic understanding of CD28 function into immunomodulatory therapeutics has been uneven, with both successes and failures from pre-clinical studies. T-cell-specific surface glycoprotein CD28

Product Categories/Family for CD28 active protein

Proteins; Enzymes; Proteins & Enzymes (A-Z); Cytokines; Growth Factors & Hormones; CDs; Human cell expressed recombinant proteins

NCBI and Uniprot Product Information

• NCBI GI #: 340545508
• NCBI GeneID: 940
• NCBI Accession #: NP_001230006.1
• NCBI GenBank Nucleotide #: NM_001243077.1
• UniProt Accession #: P10747; Q13964; Q52M23; Q8NI54; Q8NI55; Q8NI56; Q8WXJ2; Q9BYV0; A8KAC1
• Molecular Weight: 25,066 Da
• NCBI Official Full Name: T-cell-specific surface glycoprotein CD28 isoform 2
• NCBI Official Synonym Full Names: CD28 molecule
• NCBI Official Symbol: CD28
• NCBI Official Synonym Symbols: Tp44
• NCBI Protein Information: T-cell-specific surface glycoprotein CD28; CD28 antigen
• UniProt Protein Name: T-cell-specific surface glycoprotein CD28
• Protein Family: T-cell-specific surface glycoprotein
• UniProt Gene Name: CD28
• UniProt Entry Name: CD28_HUMAN

NCBI Description

The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]

Uniprot Description

CD28: a type I plasma membrane protein Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Binds to B7-1 and -2. Expressed in T cells and plasma cells, but not in less mature B cells. Six splice-variant isoforms have been described.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: integral to plasma membrane; plasma membrane; immunological synapse; cytosol; external side of plasma membrane

Molecular Function: identical protein binding; protein binding; SH3/SH2 adaptor activity; protease binding; coreceptor activity

Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of translation; nerve growth factor receptor signaling pathway; viral reproduction; positive regulation of interleukin-2 biosynthetic process; cytokine biosynthetic process; T cell receptor signaling pathway; positive regulation of inflammatory response to antigenic stimulus; cell surface receptor linked signal transduction; positive regulation of T cell proliferation; regulatory T cell differentiation; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of viral genome replication; positive regulation of mitosis; negative thymic T cell selection; regulation of defense response to virus by virus; humoral immune response; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; T cell costimulation; innate immune response; positive regulation of alpha-beta T cell proliferation; positive regulation of transcription from RNA polymerase II promoter