Carboxypeptidase E/CPE Active Protein | CPE active protein

Carboxypeptidase E/CPE Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8119983

• Purity: >90% as determined by SDS-PAGE
• Synonyms: Carboxypeptidase E/CPE; Carboxypeptidase E/CPE Protein; Human; Recombinant (His Tag); Human Carboxypeptidase E/CPE Protein (His Tag); carboxypeptidase E; CPE active protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser453

• Species: Human
• Activity: Measured by its ability to cleave a peptide substrate, benzoyl-AR-OH. The product, Arg, reacted with orthophthaldialdehyde (OPA) to form a fluorescent molecule . The specific activity is >12,000pmoles/min/ug.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 26
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human carboxypeptidase E (CPE) precursor (NP_001864.1)(Met 1-Ser 453) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CPE active protein

Background: Carboxypeptidase E (CPE), also known as Carboxypeptidase H, is a peripheral membrane protein and a zinc metallocarboxypeptidase, and the conversion of proCPE into CPE occurs primarily in secretory vesicles. The active form of CPE cleaves C-terminal amino acid residues of the peptide, and is thus involved in the biosynthesis of peptide hormones and neurotransmitters including insulin, enkephalin, etc. The enzymatic activity is enhanced by millimolar concentrations of Co2+. It has also been proposed that membrane-associated carboxypeptidase E acts as a sorting receptor for targeting regulated secretory proteins which are mostly prohormones and neuropeptides in the trans-Golgi network of the pituitary and in secretory granules into the secretory pathway.Its interaction with glycosphingolipid-cholesterol rafts at the TGN facilitates the targeting. Mutations in this gene are implicated in type II diabetes due to impaired glucose clearance and insulin resistance.

References

Manser, E. et al., 1990, Biochem. J. 267: 517-525. Cool, D.R. et al., 1997, Cell. 88: 73-83. Song, L. and Fricker, L. 1995, J. Neurochem. 65: 444-453. Dhanvantari,S. et al., 2000, J. Biol. Chem. 275: 29887-29893. Jeffrey, K.D. et al., 2008, Proc. Natl. Acad. Sci. U.S.A. 105: 8452-8457

NCBI and Uniprot Product Information

• NCBI GI #: 4503009
• NCBI GeneID: 1363
• NCBI Accession #: NP_001864.1
• NCBI GenBank Nucleotide #: NM_001873.2
• UniProt Accession #: P16870; Q9UIU9; A8K4N1; B3KR42; B4DFN4; D3DP33
• Molecular Weight: 53,151 Da
• NCBI Official Full Name: carboxypeptidase E preproprotein
• NCBI Official Synonym Full Names: carboxypeptidase E
• NCBI Official Symbol: CPE
• NCBI Protein Information: carboxypeptidase E; CPH; carboxypeptidase H; enkephalin convertase; prohormone-processing carboxypeptidase; insulin granule-associated carboxypeptidase; cobalt-stimulated chromaffin granule carboxypeptidase
• UniProt Protein Name: Carboxypeptidase E
• Protein Family: Carboxypeptidase
• UniProt Gene Name: CPE
• UniProt Synonym Gene Names: CPE; CPH
• UniProt Entry Name: CBPE_HUMAN

NCBI Description

This gene encodes a carboxypeptidase that cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. It is a peripheral membrane protein. The protein specifically binds regulated secretory pathway proteins, including prohormones, but not constitutively secreted proteins. Mutations in this gene are implicated in type II diabetes. [provided by RefSeq, Jul 2008]

Uniprot Description

CPE: Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Protease; EC 3.4.17.10; Secreted

Chromosomal Location of Human Ortholog: 4q32.3

Cellular Component: Golgi apparatus; secretory granule membrane; plasma membrane; nucleus

Molecular Function: carboxypeptidase activity; zinc ion binding; neurexin binding; cell adhesion molecule binding; metallocarboxypeptidase activity

Biological Process: insulin processing; cellular protein metabolic process; metabolic process; neuropeptide signaling pathway; protein modification process; proteolysis