Rabbit anti-Human, Mouse CPE Polyclonal Antibody | anti-CPE antibody

CPE, CT (Carboxypeptidase E, CPE, Carboxypeptidase H, CPH, Enkephalin Convertase, Prohormone-processing Carboxypeptidase)

Cat. Num. AAA6003301

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Purified; Purified by ammonium sulfate precipitation.
• Synonyms: CPE; Polyclonal Antibody; CT (Carboxypeptidase E; Carboxypeptidase H; CPH; Enkephalin Convertase; Prohormone-processing Carboxypeptidase); Anti -CPE; anti-CPE antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CPE. Species Crossreactivity: mouse.
• Purity/Purification: Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-CPE antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:50-1:100
• Immunogen: Synthetic peptide selected from the C-terminal region of human CPE (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CPE antibody (C-term) in mouse cerebellum tissue lysates (35ug/lane). CPE (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of CPE antibody (C-term) in Hela cell line lysates (35ug/lane). CPE (arrow) was detected using the purified Pab.)

Related Product Information for anti-CPE antibody

CPE is a carboxypeptidase that cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. It is a peripheral membrane protein. The protein specifically binds regulated secretory pathway proteins, including prohormones, but not constitutively secreted proteins. Mutations in CPE gene are implicated in type II diabetes.

Product Categories/Family for anti-CPE antibody

Antibodies; Abs to Enzymes; Carboxypeptidase

NCBI and Uniprot Product Information

• NCBI GI #: 4503009
• NCBI GeneID: 1363
• NCBI Accession #: NP_001864.1
• NCBI GenBank Nucleotide #: NM_001873.2
• UniProt Accession #: P16870; Q9UIU9; A8K4N1; B3KR42; B4DFN4; D3DP33
• Molecular Weight: 53,151 Da
• NCBI Official Full Name: carboxypeptidase E preproprotein
• NCBI Official Synonym Full Names: carboxypeptidase E
• NCBI Official Symbol: CPE
• NCBI Protein Information: carboxypeptidase E; CPH; carboxypeptidase H; enkephalin convertase; prohormone-processing carboxypeptidase; insulin granule-associated carboxypeptidase; cobalt-stimulated chromaffin granule carboxypeptidase
• UniProt Protein Name: Carboxypeptidase E
• Protein Family: Carboxypeptidase
• UniProt Gene Name: CPE
• UniProt Synonym Gene Names: CPE; CPH
• UniProt Entry Name: CBPE_HUMAN

NCBI Description

This gene encodes a carboxypeptidase that cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. It is a peripheral membrane protein. The protein specifically binds regulated secretory pathway proteins, including prohormones, but not constitutively secreted proteins. Mutations in this gene are implicated in type II diabetes. [provided by RefSeq, Jul 2008]

Uniprot Description

CPE: Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Protease; EC 3.4.17.10; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q32.3

Cellular Component: Golgi apparatus; secretory granule membrane; plasma membrane; nucleus

Molecular Function: carboxypeptidase activity; zinc ion binding; neurexin binding; cell adhesion molecule binding; metallocarboxypeptidase activity

Biological Process: insulin processing; cellular protein metabolic process; neuropeptide signaling pathway; metabolic process; protein modification process; proteolysis