Carboxypeptidase E / CPE Recombinant Protein | CPE recombinant protein

Recombinant Human Carboxypeptidase E / CPE Protein (His tag)

Cat. Num. AAA2546547

• Gene Names: CPE; CPH
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: Carboxypeptidase E / CPE; Recombinant Human Carboxypeptidase E / CPE Protein (His tag); CPE; CPE recombinant protein

• Host: Human Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 476

• Application Notes: The recombinant human CPE comprises 439 amino acids and predicts a molecular mass of 49.4 kDa. As a result of glycosylation, the apparent molecular mass of rhCPE is approximately 53 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Glu 26
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CPE recombinant protein

Background: Human carboxypeptidase E (CPE), also known as Carboxypeptidase H, is a peripheral membrane protein and a zinc metallocarboxypeptidase, and the conversion of proCPE into CPE occurs primarily in secretory vesicles. The active form of CPE cleaves C-terminal amino acid residues of the peptide, and is thus involved in the biosynthesis of peptide hormones and neurotransmitters including insulin, enkephalin, etc. The enzymatic activity is enhanced by millimolar concentrations of Co2+. It has also been proposed that membrane-associated carboxypeptidase E acts as a sorting receptor for targeting regulated secretory proteins which are mostly prohormones and neuropeptides in the trans-Golgi network of the pituitary and in secretory granules into the secretory pathway.Its interaction with glycosphingolipid-cholesterol rafts at the TGN facilitates the targeting. Mutations in this gene are implicated in type I I diabetes due to impaired glucose clearance and insulin resistance.

Description: A DNA sequence encoding the human carboxypeptidase E (CPE) precursor (NP_001864.1) (Met 1-Ser 453) was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 4503009
• NCBI GeneID: 1363
• NCBI Accession #: NP_001864.1
• NCBI GenBank Nucleotide #: NM_001873.3
• UniProt Accession #: P16870; Q9UIU9; A8K4N1; B3KR42; B4DFN4; D3DP33
• Molecular Weight: 49,886 Da
• NCBI Official Full Name: carboxypeptidase E preproprotein
• NCBI Official Synonym Full Names: carboxypeptidase E
• NCBI Official Symbol: CPE
• NCBI Official Synonym Symbols: CPH
• NCBI Protein Information: carboxypeptidase E
• UniProt Protein Name: Carboxypeptidase E
• Protein Family: Carboxypeptidase
• UniProt Gene Name: CPE
• UniProt Synonym Gene Names: CPE; CPH

NCBI Description

This gene encodes a member of the M14 family of metallocarboxypeptidases. The encoded preproprotein is proteolytically processed to generate the mature peptidase. This peripheral membrane protein cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. This protein may also function independently of its peptidase activity, as a neurotrophic factor that promotes neuronal survival, and as a sorting receptor that binds to regulated secretory pathway proteins, including prohormones. Mutations in this gene are implicated in type 2 diabetes. [provided by RefSeq, Nov 2015]

Uniprot Description

CPE: Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.17.10; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q32.3

Cellular Component: cell soma; extracellular space; Golgi apparatus; plasma membrane; secretory granule; secretory granule membrane

Molecular Function: carboxypeptidase activity; cell adhesion molecule binding; metallocarboxypeptidase activity; neurexin binding; serine carboxypeptidase activity

Biological Process: insulin processing; metabolic process; Wnt receptor signaling pathway