Rabbit anti-Human GAR1 Polyclonal Antibody | anti-GAR1 antibody

GAR1, ID (GAR1, NOLA1, H/ACA ribonucleoprotein complex subunit 1, Nucleolar protein family A member 1, snoRNP protein GAR1) (FITC)

Cat. Num. AAA6301250

• Gene Names: GAR1; NOLA1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GAR1; Polyclonal Antibody; ID (GAR1; NOLA1; H/ACA ribonucleoprotein complex subunit 1; Nucleolar protein family A member 1; snoRNP protein GAR1) (FITC); snoRNP protein GAR1; anti-GAR1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-GAR1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GAR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 85-114 amino acids from the Central region of human GAR1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GAR1 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the GAR1 antibody detected the GAR1 protein (arrow).)

Product Categories/Family for anti-GAR1 antibody

Antibodies; Nuclear Proteins

References

Kalsi, G., et al. Hum. Mol. Genet. 19(12):2497-2506(2010). Hamma, T., et al. J. Biol. Chem. 285(2):805-809(2010). Pigullo, S., et al. Pediatr Blood Cancer 52(3):376-378(2009). Vasan, R.S., et al. BMC Med. Genet. 8 SUPPL 1, S2 (2007): Andersen, J.S., et al. Nature 433(7021):77-83(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 15011916
• NCBI GeneID: 54433
• NCBI Accession #: NP_127460.1
• NCBI GenBank Nucleotide #: NM_032993.2
• UniProt Accession #: Q9NY12; Q5MJQ2
• Molecular Weight: 22,348 Da
• NCBI Official Full Name: H/ACA ribonucleoprotein complex subunit 1
• NCBI Official Synonym Full Names: GAR1 ribonucleoprotein homolog (yeast)
• NCBI Official Symbol: GAR1
• NCBI Official Synonym Symbols: NOLA1
• NCBI Protein Information: H/ACA ribonucleoprotein complex subunit 1; OTTHUMP00000219723; OTTHUMP00000219724; OTTHUMP00000219725; snoRNP protein GAR1; nucleolar protein family A member 1; nucleolar protein family A, member 1 (H/ACA small nucleolar RNPs)
• UniProt Protein Name: H/ACA ribonucleoprotein complex subunit 1
• Protein Family: D-galacturonate reductase
• UniProt Gene Name: GAR1
• UniProt Synonym Gene Names: NOLA1
• UniProt Entry Name: GAR1_HUMAN

NCBI Description

This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the DKC1, NOLA2 and NOLA3 proteins. These four H/ACA snoRNP proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. These four H/ACA snoRNP proteins are also components of the telomerase complex. The encoded protein of this gene contains two glycine- and arginine-rich domains and is related to Saccharomyces cerevisiae Gar1p. Two splice variants have been found for this gene. [provided by RefSeq]

Uniprot Description

NOLA1: Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine (psi) residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme. Belongs to the GAR1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; RNA-binding

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: Cajal body; nucleolus; nucleus

Molecular Function: cation channel activity

Biological Process: pseudouridine synthesis; cation transport; rRNA processing