Testing Data
CCL6 active protein
Human CellExp CCL6, mouse recombinant
Gene Names
Ccl6; c10; MRP-1; Scya6
Applications
SDS-Page
Purity
>=95%
Synonyms
CCL6; Human CellExp CCL6; mouse recombinant; SCYA6; C10; MRP-1; CCL6 active protein
Host
HEK 293 cells
Purity/Purification
>=95%
Form/Format
Lyophilized from 0.22 mum filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Appearance: Lyophilized powder
Appearance: Lyophilized powder
Sequence Length
116
Applicable Applications for CCL6 active protein
SDS-PAGE; HPLC
Solubility/Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
Endotoxin Level
< 0.1 ng/mug of protein (<1EU/mug).
Activity (Specifications/test method)
Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR1.The ED50 for this effect is typically 0.01-0.15 mug/mL.
Biological Activity
Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR1.The ED50 for this effect is typically 0.01-0.15 mug/mL.
Handling
Centrifuge the vial prior to opening.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Testing Data
Testing Data
Related Product Information for CCL6 active protein
Background: Chemokine (C-C motif) ligand 6 (CCL6), a member of the CC chemokine family, was initially identified as a transcript that is induced in bone marrow cells upon stimulation with GM-CSF. CCL6 has only been identified in rodents. A human homologue for mouse CCL6 has not been identified. The mCCL6 cDNA encodes a 116 amino acid residue precursor protein with a hydrophobic signal peptide that is cleaved to yield a 95 amino acid residue mature protein. In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, where it is strongly induced upon GM-CSF stimulation. Mouse CCL6 expression was also shown to be strongly induced by IL-4 in mouse macrophages and by cytokine IL-13 in mouse lung. Mouse CCL6 is located on chromosome 11. The cell surface receptor for CCL6 is believed to be the chemokine receptor CCR1.
Product Categories/Family for CCL6 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
This protein is fused with a C-terminal 6 × His tag, has a calculated MW of 11.4 kDa expressed. The predicted N-terminus is Gly22. Protein migrates as 14 kDa in reduced SDS-PAGE.
NCBI Official Full Name
C-C motif chemokine 6
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 6
NCBI Official Symbol
Ccl6
NCBI Official Synonym Symbols
c10; MRP-1; Scya6
NCBI Protein Information
C-C motif chemokine 6
UniProt Protein Name
C-C motif chemokine 6
Protein Family
UniProt Gene Name
Ccl6
UniProt Synonym Gene Names
C10; Scya6
UniProt Entry Name
CCL6_MOUSE
Uniprot Description
CCL6: Shows chemotactic activity for monocytes, resting T- lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are more potent chemoattractants than the small-inducible cytokine A23. Belongs to the intercrine beta (chemokine CC) family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis
Cellular Component: extracellular space; extracellular region
Molecular Function: chemokine activity; cytokine activity
Biological Process: immune response; chemotaxis
Research Articles on CCL6
Similar Products
Product Notes
The CCL6 ccl6 (Catalog #AAA845417) is an Active Protein produced from HEK 293 cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CCL6 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE; HPLC. Researchers should empirically determine the suitability of the CCL6 ccl6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCL6, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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