CCL6 recombinant protein

CCL6 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122478

• Gene Names: Ccl6; c10; MRP-1; Scya6
• Purity: >90% as determined by SDS-PAGE
• Synonyms: CCL6; CCL6 Protein; Mouse; Recombinant (His Tag); Mouse CCL6/C-C motif ligand 6 Protein (His Tag); c10 Protein; MRP-1 Protein; RP23-320E6.4 Protein; Scya6 Protein; chemokine (C-C motif) ligand 6; CCL6 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala116

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gly 22
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse CCL6 (P27784)(Met 1-Ala 116) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CCL6 recombinant protein

Background: Chemokine (C-C motif) ligand 6 (CCL6), also known as C-C chemokine C10 has only been identified in rodents, which is a small cytokine belonging to the CC chemokine family, beta-chemokine subfamily. C-C chemokine C10 is involved in the chronic stages of host defense reactions. C10 chemokine rapidly promotes disease resolution in the cecal ligation and puncture (CLP) model through its direct effects on the cellular events critically involved in host defense during septic peritonitis. CCL6 appears to contribute to the macrophage infiltration that is independent of other CC chemokines. C10 is a prominent chemokine expressed in the central nervous system in experimental inflammatory demyelinating disease, also acts as a potent chemotactic factor for the migration of these leukocytes to the brain. CCL6 may be a mediator released by microglia for cell-cell communication under physiological as well as pathological conditions of CNS. Additionally, the chemokine CCL6 may alter tumor behavior by relieving its growth factor dependency and by promoting invasiveness as a result of local tissue apoptosis.

References

Asensio VC, et al. (1999) C10 is a novel chemokine expressed in experimental inflammatory demyelinating disorders that promotes recruitment of macrophages to the central nervous system. Am J Pathol. 154(4): 1181-91.Steinhauser ML, et al. (2000) Chemokine C10 promotes disease resolution and survival in an experimental model of bacterial sepsis. Infect Immun. 68(11): 6108-14.Yi F, et al. (2003) The CCL6 chemokine is differentially regulated by c-Myc and L-Myc, and promotes tumorigenesis and metastasis. Cancer Res. 63(11): 2923-32.LaFleur AM, et al. (2004) Role of CC chemokine CCL6/C10 as a monocyte chemoattractant in a murine acute peritonitis. Mediators Inflamm. 13(5-6): 349-55.Kanno M, et al. (2005) Functional expression of CCL6 by rat microglia: a possible role of CCL6 in cell-cell communication. J Neuroimmunol. 167(1-2): 72-80.

NCBI and Uniprot Product Information

• NCBI GI #: 6677883
• NCBI GeneID: 20305
• NCBI Accession #: NP_033165.1
• NCBI GenBank Nucleotide #: NM_009139.3
• UniProt Accession #: P27784; Q3U3W3; Q5QNW1; Q99M24; Q9D830
• Molecular Weight: 12,984 Da
• NCBI Official Full Name: C-C motif chemokine 6
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 6
• NCBI Official Symbol: Ccl6
• NCBI Official Synonym Symbols: c10; MRP-1; Scya6
• NCBI Protein Information: C-C motif chemokine 6; CC chemokine C10; small inducible cytokine A6; small-inducible cytokine A6
• UniProt Protein Name: C-C motif chemokine 6
• Protein Family: C-C motif chemokine
• UniProt Gene Name: Ccl6
• UniProt Synonym Gene Names: C10; Scya6
• UniProt Entry Name: CCL6_MOUSE

Uniprot Description

CCL6: Shows chemotactic activity for monocytes, resting T- lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are more potent chemoattractants than the small-inducible cytokine A23. Belongs to the intercrine beta (chemokine CC) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine activity; cytokine activity

Biological Process: immune response; chemotaxis