IL-1 Alpha Active Protein | IL-1a active protein

Human CellExp IL-1 Alpha, human recombinant

Cat. Num. AAA844478

• Gene Names: IL1A; IL1; IL-1A; IL1F1; IL1-ALPHA
• Applications: SDS-Page
• Purity: >=97%
• Synonyms: IL-1 Alpha; Human CellExp IL-1 Alpha; human recombinant; IL1A; IL-1A; IL1; IL1-ALPHA; IL1ALPHA; IL1F1; IL-1alpha; IL1alpha; Interleukin-1 alpha; Interleukin-1; Interleukin; IL-1a active protein

• Host: HEK 293 cells
• Purity/Purification: >=97%
• Form/Format: Lyophilized from 0.22 mum filtered solution in in PBS, pH 7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.; Appearance: Lyophilized powder
• Sequence Length: 271

• Applicable Applications for IL-1a active protein: SDS-PAGE
• Solubility/Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
• Endotoxin Level: < 0.1 ng/mug of protein (<1EU/mug) by LAL method
• Activity (Specifications/test method): The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse D10S cells. The ED50 <1pg/ml, corresponding to a specific activity of >1X10^9 Unit/mg.
• Biological Activity: The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse D10S cells. The ED50 <1pg/ml, corresponding to a specific activity of >1X10^9 Unit/mg.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for IL-1a active protein

Background: Interleukin-1 alpha (IL1a) is also known as IL-1A, IL1, IL1-ALPHA, IL1F1, and is a cytokine of the interleukin-1 family. IL1a possesses a wide spectrum of metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. IL-1alpha is constitutively produced by epithelial cells. It is found in substantial amounts in normal human epidermis and is distributed in a 1:1 ratio between living epidermal cells and stratum corneum. The constitutive production of large amounts of IL-1alpha precursor by healthy epidermal keratinocytes interfere with the important role of IL-1alpha in immune responses, assuming skin as a barrier, which prevents the entry of pathogenic microorganisms into the body. In vitro, IL-1alpha possesses biological effect on cells in the picomolar to femtomolar range. In vivo, shortly after an onset of an infection into organism, IL-1alpha activates a set of immune system response processes.

Product Categories/Family for IL-1a active protein

Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteins (NEW) Cytokines; Growth Factors & Hormones; InterleukinsCytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Human cell expressed recom

NCBI and Uniprot Product Information

• NCBI GI #: 27894330
• NCBI GeneID: 3552
• NCBI Accession #: NP_000566.3
• NCBI GenBank Nucleotide #: NM_000575.3
• UniProt Accession #: P01583; Q53QF9; Q7RU02
• Molecular Weight: Calculated MW of 18.0 kDa with no tag. DTT-reduced protein migrates as 22 kDa. in SDS-PAGE
• NCBI Official Full Name: interleukin-1 alpha proprotein
• NCBI Official Synonym Full Names: interleukin 1, alpha
• NCBI Official Symbol: IL1A
• NCBI Official Synonym Symbols: IL1; IL-1A; IL1F1; IL1-ALPHA
• NCBI Protein Information: interleukin-1 alpha
• UniProt Protein Name: Interleukin-1 alpha
• UniProt Gene Name: IL1A
• UniProt Synonym Gene Names: IL1F1; IL-1 alpha
• UniProt Entry Name: IL1A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008]

Uniprot Description

IL1A: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Belongs to the IL-1 family.

Protein type: Cytokine; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q14

Cellular Component: extracellular space; extracellular region; cytosol

Molecular Function: protein binding; copper ion binding; interleukin-1 receptor binding; cytokine activity

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of mitosis; apoptosis; positive regulation of interleukin-2 biosynthetic process; cytokine and chemokine mediated signaling pathway; germ cell programmed cell death; positive regulation of JNK cascade; fever; cell proliferation; negative regulation of cell proliferation; connective tissue replacement during inflammatory response; positive regulation of angiogenesis; response to copper ion; immune response; positive regulation of transcription from RNA polymerase II promoter; inflammatory response; positive regulation of cytokine secretion