HGFR/c-MET Active Protein | MET active protein

Human CellExp HGFR / c-MET, human recombinant

Cat. Num. AAA844448

• Gene Names: MET; HGFR; AUTS9; RCCP2; c-Met; DFNB97
• Applications: SDS-Page
• Purity: >=95%
• Synonyms: HGFR/c-MET; Human CellExp HGFR / c-MET; human recombinant; MET; AUTS9; HGFR; RCCP2; c-Met; MET active protein

• Host: HEK293 cells
• Purity/Purification: >=95%
• Form/Format: Lyophilized from 0.22 mum filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.; Appearance: Lyophilized
• Sequence Length: 1390

• Applicable Applications for MET active protein: SDS-PAGE
• Solubility/Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
• Endotoxin Level: <1 EU/mug by LAL method
• Biological Activity: Measured by its binding ability in a functional ELISA Immobilized recombinant human HGF at 10 mug/ml (100 mul/well) can bind biotinylated c-Met. The EC50 of biotinylated c-Met is 2.52 mug/ml.
• Results: Measured by its binding ability in a functional ELISA Immobilized recombinant human HGF at 10 mug/ml (100 mul/well) can bind biotinylated c-Met. The EC50 of biotinylated c-Met is 2.52 mug/ml.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for MET active protein

Background: Hepatocyte growth factor receptor (HGFR), also known as mesenchymal-epithelial transition factor (MET), c-Met, and is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGFR protein possesses tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. HGFR is normally expressed by cells of epithelial origin, while expression of HGF is restricted to cells of mesenchymal origin. Upon HGF stimulation, HGFR induces several biological responses that collectively give rise to a program known as invasive growth. Abnormal HGFR activation in cancer correlates with poor prognosis, where aberrantly active HGFR triggers tumor growth, formation of new blood vessels (angiogenesis) that supply the tumor with nutrients, and cancer spread to other organs (metastasis). HGFR is deregulated in many types of human malignancies, including cancers of kidney, liver, stomach, breast, and brain. Normally, only stem cells and progenitor cells express HGFR, However, cancer stem cells are thought to hijack the ability of normal stem cells to express HGFR, and thus become the cause of cancer persistence and spread to other sites in the body. Various mutations in the HGFR gene are associated with papillary renal carcinoma. HGFR mediates a complex program known as invasive growth. Activation of HGFR triggers mitogenesis, and morphogenesis.

Product Categories/Family for MET active protein

Proteins and Enzymes; Human cell expressed recombinant proteinsCytokines; Growth Factors & Hormones; Human cell expressed recombinant proteinsCytokines; Growth Factors & Hormones; HGFsCytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 42741655
• NCBI GeneID: 4233
• NCBI Accession #: NP_000236.2
• NCBI GenBank Nucleotide #: NM_000245.2
• UniProt Accession #: P08581; O60366; Q12875; Q9UDX7; Q9UPL8; A1L467; B5A932; E7EQ94
• Molecular Weight: This protein is fused with C-terminal 8×His tag. The mature form of HGFR is a disulfide-linked heterodimer composed of proteolytically cleaved alpha and beta chain. Each alpha and beta chain has a calculated MW of 32.5 kDa (alpha chain) and 60 kDa (beta c
• NCBI Official Full Name: hepatocyte growth factor receptor isoform b
• NCBI Official Synonym Full Names: MET proto-oncogene, receptor tyrosine kinase
• NCBI Official Symbol: MET
• NCBI Official Synonym Symbols: HGFR; AUTS9; RCCP2; c-Met; DFNB97
• NCBI Protein Information: hepatocyte growth factor receptor
• UniProt Protein Name: Hepatocyte growth factor receptor
• Protein Family: Hepatocyte growth factor receptor
• UniProt Gene Name: MET
• UniProt Synonym Gene Names: HGF receptor; SF receptor
• UniProt Entry Name: MET_HUMAN

NCBI Description

The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Met: a proto-oncogenic receptor tyrosine kinase with high affinity for hepatocyte growth factor. The primary single chain precursor protein is post-translationally cleaved to produce the 45 kDa alpha- and 145 kDa beta-subunits, which are disulfide linked to form the mature receptor. Ligand-binding induces autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl and PI3 kinase. Activating point mutations cause hereditary papillary renal carcinoma. Mutations also seen in sporadic renal cell carcinoma and childhood hepatocellular carcinoma. Upregulation in carcinomas and sarcomas correlates with metastasis and poor outcome. Some gastric carcinomas harbor a translocation that creates an activated TPR-Met fusion protein. A small molecule inhibitor (PHA-665752) shows an effect in gastric carcinoma xenografts. Inhibitors: SU11274, PHA-665752, mAbs. Two alternatively spliced human isoforms have been reported.

Protein type: Kinase, protein; Membrane protein, integral; Oncoprotein; Protein kinase, tyrosine (receptor); EC 2.7.10.1; Protein kinase, TK; TK group; Met family

Chromosomal Location of Human Ortholog: 7q31

Cellular Component: cell surface; integral to plasma membrane; basal plasma membrane; extracellular region; plasma membrane; integral to membrane

Molecular Function: protein binding; protein-tyrosine kinase activity; protein phosphatase binding; hepatocyte growth factor receptor activity; ATP binding

Biological Process: axon guidance; skeletal muscle development; peptidyl-tyrosine phosphorylation; activation of MAPK activity; protein amino acid autophosphorylation; myoblast proliferation; myotube differentiation; muscle cell migration; hepatocyte growth factor receptor signaling pathway; glucose homeostasis; liver development; signal transduction; cell proliferation; cell surface receptor linked signal transduction; positive chemotaxis; branching morphogenesis of a tube; adult behavior; positive regulation of transcription from RNA polymerase II promoter; brain development; endothelial cell morphogenesis; placenta development

Disease: Hepatocellular Carcinoma; Renal Cell Carcinoma, Papillary, 1