HDAC11 active protein

Human CellExp HDAC11, Human Recombinant, Active

Cat. Num. AAA849894

• Gene Names: HDAC11; HD11
• Purity: >=80% by SEC
• Synonyms: HDAC11; Human CellExp HDAC11; Human Recombinant; Active; HD11; Histone Deacetylase 11; HDAC11 active protein

• Host: HEK293 cells
• Purity/Purification: >=80% by SEC
• Form/Format: Liquid. In 30mM HEPES, 140mM NaCl, 10mM KCl, 3% glycerol, 0.25mM TCEP, pH7.6

• Gene Source: Human
• Biological Activity: Deacetylation activity was determined using a myristoylated substrate. Reactions were carried outin a 384-well plate in 20 uL ofreaction buffer comprising of 50 mM HEPES, 140 mM NaCl, 10 mM KCl, 1 mg/ml bovine serum albumin (BSA), and 1 mM TCEP, pH 7.4, for 30 min at 37 degree C. Thereaction was stopped by addition of 20ul of trypsin solution (2mg/mltrypsin) and thefluorescence signal of released AMC was quantified using a fluorometer. The enzymekinetic values were calculated by non-linear regression analysis.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Store at -80 degree C; Thaw on ice, and aliquot into smaller working quantities to avoid multiple freeze/thaw cycles.; Shipping: Dry Ice; Shelf life: ~12 months

Testing Data

Related Product Information for HDAC11 active protein

HDAC11 is a 39kDa protein and belongs to the Histone Deacetylase Class IV subfamily. The enzyme is primarily localized in the nucleus and shows high expression in specific tissues like brain, heart, skeletal muscle and kidney. It is the newest member of the HDAC family and hence very little is known about its functional role. Initial studies identified HDAC11 as a negative regulator of IL-10 production in APC cells. A recent study shows that HDAC11 is the most efficient fatty acid deacetylase of the HDAC family with catalytic efficiencies towards dodecanoylated and myristoylated substrates exceeding 70,000 M-1 s -1. Increasing body of evidence indicate a possible involvement of HDAC11 in diverse pathophysiological processes including immunomodulation, cancer pathogenesis, cellular differentiation etc

Product Categories/Family for HDAC11 active protein

Cancer Research; Epigenetic Regulation; Eraser Enzymes; Histone Deacetylases (HDACs); HDAC Enzymes & Substrates

NCBI and Uniprot Product Information

• NCBI GI #: 217272877
• NCBI GeneID: 79885
• NCBI Accession #: NP_079103.2
• NCBI GenBank Nucleotide #: NM_024827.3
• UniProt Accession #: Q96DB2; Q9H6I7; Q9H6X3; Q9NTC9; B4DDK1
• Molecular Weight: 33,124 Da
• NCBI Official Full Name: histone deacetylase 11 isoform 1
• NCBI Official Synonym Full Names: histone deacetylase 11
• NCBI Official Symbol: HDAC11
• NCBI Official Synonym Symbols: HD11
• NCBI Protein Information: histone deacetylase 11
• UniProt Protein Name: Histone deacetylase 11
• Protein Family: Histone deacetylase
• UniProt Gene Name: HDAC11
• UniProt Synonym Gene Names: HD11

NCBI Description

This gene encodes a class IV histone deacetylase. The encoded protein is localized to the nucleus and may be involved in regulating the expression of interleukin 10. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Apr 2009]

Uniprot Description

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.