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SDS-PAGE ( SDS-PAGE (12%):purified E2 (HCV) protein (aa 383-663), subtype 1a)

HCV E2 protein Recombinant Protein

HCV E2 protein (aa 383-663), Subtype 1a

Applications
Western Blot, ELISA
Purity
>= 95% purity
Synonyms
HCV E2 protein; HCV E2 protein (aa 383-663); Subtype 1a; subtype 1a; HCV E2 PROTEIN; HCV E2 protein recombinant protein
Ordering
For Research Use Only!
Host
293 cell culture
Purity/Purification
>= 95% purity
Concentration
1 ug/ul in PBS with 20% glycerol and 0.1% sodium azide. (varies by lot)
Sequence
AETHVTGGSAGRTTAGLVGLLTPGAKQNIQLINTNGSWHINSTALNCNESLNTGWLAGLFYQHKFNSSGCPERLASCRRLTDFAQGWGPISYANGSGLDERPYCWHYPPRPCGIVPAKSVCGPVYCFPSPVVVGTTDRSGAPTYSWGANDTDVFVLNNTRPPLGNWFGCTWMNSTGFTKVCGAPPCVIGGVGNNTLLCPTDCFRKHPEATYSRCGSGPWITPRCMVDYPYRLWHYPCTINYTIFKVRMYVGGVERLEAACNWTRGERCDLEDRDRSELS
Applicable Applications for HCV E2 protein recombinant protein
Western Blot (WB), Antigen, ELISA (EIA)
Note
Viral protein purified from 293 cell culture (heavily N-glycosylated)
Endotoxin Level
<0.01 EU per 1 ug of the protein by LAL test
Viral Protein
C-terminal 6xHis tagged HCV E2 protein (amino acid 383-663) (Genebank No. AF009606), subtype 1a
Preparation and Storage
Store at -20 degree C. Stable for 3-months from the date of shipment when kept at 4 degree C. Non-hazardous. No MSDS required.

SDS-PAGE

( SDS-PAGE (12%):purified E2 (HCV) protein (aa 383-663), subtype 1a)

SDS-PAGE ( SDS-PAGE (12%):purified E2 (HCV) protein (aa 383-663), subtype 1a)
Related Product Information for HCV E2 protein recombinant protein
Description: 6xHis tagged HCV E2 protein (aa 383-663)(subtype 1a) expressed in 293 cells (Genebank Accession No. AF009606)

Viral protein purified from 293 cell culture (heavily N-glycosylated)

Introduction: The hepatitis C virus (HCV) is a small, enveloped, single-stranded, positive-sense RNA virus. HCV has a high rate of replication with approximately one trillion particles produced each day in an infected individual. Due to lack of proofreading by the HCV RNA polymerase, the HCV has an exceptionally high mutation rate, a factor that may help it elude the host's immune response. There are seven major genotypes of HCV with several subtypes within each genotype.
The viral RNA codes for a large polyprotein of approx. 3100 amino acids, which is posttranslationally processed by cellular and viral proteases. The N-terminus encompasses the structural proteins core and two glycoproteins (E1, E2); the C-terminus encompasses the p7 protein and the nonstructural (NS) proteins NS2, NS3, NS4A, NS4B, NS5A and NS5B.
Product Categories/Family for HCV E2 protein recombinant protein

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Product Notes

The HCV E2 protein (Catalog #AAA434232) is a Recombinant Protein produced from 293 cell culture and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HCV E2 protein can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Antigen, ELISA (EIA). Researchers should empirically determine the suitability of the HCV E2 protein for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: AETHVTGGSA GRTTAGLVGL LTPGAKQNIQ LINTNGSWHI NSTALNCNES LNTGWLAGLF YQHKFNSSGC PERLASCRRL TDFAQGWGPI SYANGSGLDE RPYCWHYPPR PCGIVPAKSV CGPVYCFPSP VVVGTTDRSG APTYSWGAND TDVFVLNNTR PPLGNWFGCT WMNSTGFTKV CGAPPCVIGG VGNNTLLCPT DCFRKHPEAT YSRCGSGPWI TPRCMVDYPY RLWHYPCTIN YTIFKVRMYV GGVERLEAAC NWTRGERCDL EDRDRSELS. It is sometimes possible for the material contained within the vial of "HCV E2 protein, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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