H5 (H5N1) (A/Indonesia/5/2005) Recombinant Protein | H5 recombinant protein

H5 (H5N1) (aa 18-530)(A/Indonesia/5/2005)

Cat. Num. AAA434009

• Applications: Western Blot, ELISA
• Purity: >95% pure by 10% SDS PAGE gel.
• Synonyms: H5 (H5N1) (A/Indonesia/5/2005); H5 (H5N1) (aa 18-530)(A/Indonesia/5/2005); glycosylated recombinant protein; HA (aa 18-530)(H5N1)(A/Indonesia/5/2005); H5 (H5N1); 6xHis tagged Hemagglutinin (A/Indonesia/5/2005) (aa 18-530); H5 recombinant protein

• Host: 293 cell culture
• Purity/Purification: >95% pure by 10% SDS PAGE gel.
• Concentration: 1ug/ul in PBS with 20% glecerol and 0.1% sodium azide (varies by lot)
• Sequence: DQICIGYHANNSTEQVDTIMEKNVTVTHAQDILEKTHNGKLCDLDGVKPLILRDCSVAGWLLGNPMCDEFINVPEWSYIVEKANPTNDLCYPGSFNDYEELKHLLSRINHFEKIQIIPKSSWSDHEASSGVSSACPYLGSPSFFRNVVWLIKKNSTYPTIKKSYNNTNQEDLLVLWGIHHPNDAAEQTRLYQNPTTYISIGTSTLNQRLVPKIATRSKVNGQSGRMEFFWTILKPNDAINFESNGNFIAPEYAYKIVKKGDSAIMKSELEYGNCNTKCQTPMGAINSSMPFHNIHPLTIGECPKYVKSNRLVLATGLRNSPQRESRRKKRGLFGAIAGFIEGGWQGMVDGWYGYHHSNEQGSGYAADKESTQKAIDGVTNKVNSIIDKMNTQFEAVGREFNNLERRIENLNKKMEDGFLDVWTYNAELLVLMENERTLDFHDSNVKNLYDKVRLQLRDNAKELGNGCFEFYHKCDNECMESIRNGTYNYPQYSEEARLKREEISGVKLESIGTYHHHHHH
• Sequence Length: 568

• Applicable Applications for H5 recombinant protein: Western Blot (WB), ELISA (EIA), Antigen, Etc.
• Application Notes: SDS-PAGE Data: purified HA (H5N1) (A/Indonesia/5/2005) protein
• Endotoxin Level: <0.01 EU per 1 ug of the protein by LAL test
• Viral Protein: C-terminal 6xHis tagged HA(H5N1) (A/Indonesia/5/2005) protein(amino acid 17-530)(GenBank accession# ABP51969). Majority of the protein exists as trimer/oligomer.
• Preparation and Storage: Store at -20 degree C. Stable for 6-months from the date of shipment when kept at 4 degree C. Non-hazardous. No MSDS required.

SDS-PAGE (SDS)

(Purified HA(H5N1) (A/Indonesia/5/2005) protein.)

Related Product Information for H5 recombinant protein

Description: 6xHis tagged H5 (H5N1) (A/Indonesia/5/2005) protein (amino acid 18-530) with a deletion at its cleaveage site (ˆ†RRRKKR) (Genebank accession# ABP51969)
Viral protein purified from 293 cell culture.
Introduction: Influenza hemagglutinin (HA) is a type of hemagglutinin found on the surface of the influenza viruses. HA is an antigenic glycoprotein, like all other hemagglutinins, it causes red blood cells to agglutinate. HA is responsible for binding the virus to the cell that is being infected. HA proteins bind to cells with sialic acid on the membranes, such as cells in the upper respiratory tract or erythrocytes.
HA is a homotrimeric integral membrane glycoprotein. HA monomer is synthesized as a single polypeptide that is subsequently cleaved into two smaller polypeptides, the HA1 and HA2 subunits. Each HA monomer consists of a long, helical chain anchored in the membrane by HA2 and topped by a large HA1 globule.

Product Categories/Family for H5 recombinant protein

Viral Recombinant Proteins; Influenza

NCBI and Uniprot Product Information

• NCBI GI #: 145284450
• NCBI Official Full Name: hemagglutinin
• UniProt Protein Name: Hemagglutinin
• Protein Family: Probable tyrosine phosphatase protein
• UniProt Gene Name: HA
• UniProt Entry Name: A5A5L7_9INFA

Uniprot Description

Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.