SDS-Page
HCV (HCV-1a) NS3 protease/helicase immunodominant region Protein Recombinant Protein | NS3 recombinant protein
Recombinant HCV (HCV-1a) NS3 protease/helicase immunodominant region Protein (aa 1356-1459, GST Tag)
Purity
>95% as determined by SDS-PAGE
Synonyms
HCV (HCV-1a) NS3 protease/helicase immunodominant region Protein; Recombinant HCV (HCV-1a) NS3 protease/helicase immunodominant region Protein (aa 1356-1459; GST Tag); NS3 Protein; HCV; NS3 recombinant protein
Host
E Coli
Purity/Purification
>95% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 50 mM Tris, 500 mM NaCl. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Sequence
Thr1356-Thr1459
Species
Hepatitis C Virus (HCV)
Tag
N-GST
Endotoxin
Please contact us for more information.
Protein Construction
A DNA sequence encoding the Hepatitis C virus (HCV)(serotype 1a, isolate H77) NS3 (NP_803144.1)(Thr1356-Thr1459) was expressed with a GST tag at the N-terminus
Preparation and Storage
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.
This product is provided as lyophilized powder which is shipped with ice packs.
This product is provided as lyophilized powder which is shipped with ice packs.
SDS-Page
SDS-Page
Related Product Information for NS3 recombinant protein
HCV NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. HCV NS3 serine protease, in association with NS4A, is responsible for the cleavages of NS3-NS4A, NS4A-NS4B, NS4B-NS5A and NS5A-NS5B. NS3/NS4A complex also prevents phosphorylation of human IRF3, thus preventing the establishment of dsRNA induced antiviral state. HCV NS3 RNA helicase binds to RNA and unwinds dsRNA in the 3' to 5' direction, and likely RNA stable secondary structure in the template strand (By similarity). Cleaves and inhibits the host antiviral protein MAVS.
Product Categories/Family for NS3 recombinant protein
NCBI and Uniprot Product Information
Molecular Weight
Mol. Mass: 37.9kDa
Protein Family
Similar Products
Product Notes
The NS3 (Catalog #AAA2569524) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Thr1356-Th r1459. It is sometimes possible for the material contained within the vial of "HCV (HCV-1a) NS3 protease/helicase immunodominant region Protein, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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